本文選題：番茄 + 番茄黃化曲葉病毒��； 參考：《園藝學(xué)報》2016年01期

【摘要】：采用番茄黃化曲葉病毒(Tomato yellow leaf curl virus,TYLCV)侵染克隆接種技術(shù)和實時熒光定量PCR方法,從TYLCV在番茄葉片內(nèi)復(fù)制繁殖的角度,系統(tǒng)研究了不同環(huán)境溫度下單個和多個抗性基因疊加對病毒復(fù)制的影響,以期為合理進行抗病基因整合,選育抗TYLCV的番茄新品種提供理論指導(dǎo)。結(jié)果顯示,(1)春季溫室栽培環(huán)境下,含Ty-1/Ty-3的番茄材料能抑制病毒復(fù)制,接種后28 d其體內(nèi)病毒含量僅是感病材料病毒含量的千分之一;秋季溫室栽培環(huán)境下,這種抑制作用降低,病毒含量與感病材料相當(dāng)。精確控溫種植的含Ty-1/Ty-3的近等基因系番茄材料中病毒的含量變化趨勢與此相同。(2)含Ty-2的番茄材料在春秋兩季栽培環(huán)境下,均表現(xiàn)出對病毒復(fù)制的抑制作用,接種后28 d其體內(nèi)病毒含量僅為感病材料病毒含量的萬分之一。(3)同時含有2個基因(Ty-1和Ty-2)和多個基因(Ty-1、Ty-2和Ty-3)的番茄材料在抑制病毒復(fù)制方面不存在累加效應(yīng)。
[Abstract]:Tomato yellow leaf curl virus (TYLCV) was used to infect tomato yellow leaf curl virus (TYLCV). The technique of clone inoculation and real-time fluorescence quantitative PCR (PCR) were used to replicate and reproduce TYLCV in tomato leaves. The effects of single or multiple resistance genes superposition on virus replication at different ambient temperatures were systematically studied in order to provide theoretical guidance for rational integration of disease resistance genes and selection of new tomato varieties resistant to TYLCV. The results showed that tomato material containing Ty-1/Ty-3 could inhibit virus replication in spring greenhouse, and the virus content in tomato was only 1/1000 of that of susceptible material 28 days after inoculation, but decreased in autumn. The virus content is equal to that of susceptible materials. The variation trend of virus content in the near-isogenic tomato with Ty-1/Ty-3 was the same as this. The tomato material with Ty-2 showed the inhibitory effect on virus replication in spring and autumn cultivation environment. 28 days after inoculation, the virus content in the tomato was only 1/10000% of that of the susceptible material.) there was no accumulative effect on the inhibition of virus replication in tomato materials containing two genes (Ty-1 and Ty-2) and several genes (Ty-1, Ty-2 and Ty-3).
【作者單位】： 中國農(nóng)業(yè)科學(xué)院蔬菜花卉研究所;
【基金】：國家自然科學(xué)基金項目(31272160) 國家‘863’計劃項目(2012AA100100) 農(nóng)業(yè)部園藝作物生物學(xué)與種質(zhì)創(chuàng)制重點實驗室項目
【分類號】：S436.412.11

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