本文選題：水稻 + ospls��； 參考：《作物學報》2016年05期

【摘要】：葉片衰老是作物葉片發(fā)育的最后階段,功能葉早衰將影響作物產量和品質,因此,研究葉片早衰的分子與生理機制對于培育耐早衰優(yōu)良品種具有重要意義。本研究利用~(60)Co-g輻射誘變秈稻N142,獲得葉片早衰突變體ospls3,其葉片早衰始于分蘗期,最先表現為葉尖變褐及葉中上部出現褐色斑點,并向葉基部蔓延而使葉片枯死。生理分析表明,野生型劍葉的葉綠素含量顯著低于倒二葉和倒三葉,而突變體的含量則分別低于野生型且依次顯著降低;野生型劍葉、倒二葉和倒三葉間的超氧化物歧化酶(SOD)活性、過氧化物酶(POD)活性、丙二醛(MDA)含量、O_2?~含量和H_2O_2含量基本不變,而突變體的這些活性和含量則依次顯著升高;野生型劍葉、倒二葉和倒三葉的可溶性蛋白含量和過氧化氫酶(CAT)活性變化不顯著,而突變體則依次降低。遺傳分析表明,ospls3突變性狀受1對隱性基因控制。借助圖位克隆技術,將該基因定位于第12染色體長臂的RM6953與RM28753之間,物理距離為294 kb,該結果為進一步克隆Os PLS3基因并研究其功能奠定了基礎。
[Abstract]:Leaf senescence is the last stage of crop leaf development, and functional leaf premature senescence will affect crop yield and quality. Therefore, it is important to study the molecular and physiological mechanism of leaf premature senescence in order to cultivate good varieties with early senescence resistance. In this study, the mutant ospls3 of leaf premature senescence was obtained by radiation mutagenesis of N142. The early senescence of leaves began at tillering stage, and the first appeared as browning of the tip and brown spots in the middle and upper part of the leaves, which spread to the base of the leaves and caused the leaves to wither and die. Physiological analysis showed that the chlorophyll content of wild flag leaf was significantly lower than that of the second leaf and the third leaf, while the content of mutant was lower than that of the wild leaf and decreased significantly in turn. The activity of superoxide dismutase (SOD), peroxidase (POD), malondialdehyde (MDA) content in the second leaf and the third leaf were basically unchanged, while the activity and content of these mutants increased significantly. The soluble protein content and catalase CAT activity of the second leaf and the third leaf did not change significantly, but the mutant decreased in turn. Genetic analysis showed that a pair of recessive genes controlled the mutant traits. With the help of map-based cloning technique, the gene was located between RM6953 and RM28753 on chromosome 12 with a physical distance of 294kb. The results laid a foundation for further cloning of Os PLS3 gene and study of its function.
【作者單位】： 浙江大學農業(yè)與生物技術學院;浙江省農業(yè)科學院;
【基金】：國家自然科學基金項目(31271691) 國家轉基因生物新品種培育重大專項(2013ZX08001-002)資助~~
【分類號】：S511

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