本文選題：何首烏飲 + 睪丸　； 參考：《解剖學(xué)報》2017年03期

【摘要】：目的探討何首烏飲對衰老大鼠睪丸生精細(xì)胞線粒體凋亡通路關(guān)鍵基因表達(dá)的影響。方法選用12月齡Wistar雄鼠45只,隨機(jī)分為3組:青年對照組、自然衰老組、何首烏飲組,每組15只,何首烏飲組和自然衰老組于16個月開始分別灌胃何首烏飲和等量蒸餾水,連續(xù)60d。青年對照組于12月齡,其余兩組于18月齡分別進(jìn)行實驗,通過Real-time PCR、免疫熒光染色和免疫印跡法,觀察各組大鼠睪丸組織線粒體凋亡通路關(guān)鍵基因DR6、BAX、Caspase-3、Cyt-C、14-3-3σ的表達(dá)變化。結(jié)果自然衰老大鼠線粒體凋亡通路關(guān)鍵基因14-3-3σ表達(dá)明顯低于青年對照組,DR6、BAX、Caspase-3、Cyt-C表達(dá)明顯高于青年對照組;而何首烏飲用藥后能明顯逆轉(zhuǎn)上述現(xiàn)象。結(jié)論何首烏飲提高衰老大鼠精子質(zhì)量與線粒體凋亡通路有關(guān)。
[Abstract]:Objective to investigate the effect of HYDU decoction on the expression of key genes in mitochondria apoptosis pathway of testicular spermatogenic cells in aging rats. Methods Forty-five 12-month-old male Wistar rats were randomly divided into three groups: young control group, natural aging group, polygonum multiflorum drink group (15 rats in each group), Radix Polygoni multiflorum group and natural aging group (n = 15). For 60 consecutive days. The young control group was 12 months old and the other two groups were 18 months old. The expression of the key gene DR6, Bax, Caspase-3 and Cyt-Con 14-3-3 蟽 was observed by Real-time, immunofluorescence staining and Western blotting. Results the expression of 14-3-3 蟽 in mitochondria apoptosis pathway in natural aging rats was significantly lower than that in young control group (P < 0.01). Conclusion the increase of sperm quality in aging rats is related to mitochondrial apoptosis pathway.
【作者單位】： 河北省中醫(yī)院藥學(xué)部;中國人民解放軍軍事交通學(xué)院九連;河北大學(xué)醫(yī)學(xué)院組織學(xué)胚胎學(xué)教研室;河北師范大學(xué)生命科學(xué)學(xué)院;
【基金】：國家自然科學(xué)基金(81673714)
【分類號】：R285.5

【參考文獻(xiàn)】

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