本文選題：鏈球菌 + 豬場�。� 參考：《畜牧與獸醫(yī)》2017年01期

【摘要】：采用微量稀釋法進(jìn)行11種獸用藥及11種人用抗菌藥的藥物敏感性檢測試驗(yàn)。結(jié)果表明:豬源和人源鏈球菌同時(shí)對8種獸用抗菌藥和10種人用抗菌藥物產(chǎn)生表觀耐藥性。針對分離菌株進(jìn)行耐藥基因檢測,選取共同耐藥率較高的3類抗菌藥物的9種主要相關(guān)基因進(jìn)行檢測,發(fā)現(xiàn)豬源與人源鏈球菌攜帶共同耐藥基因有6種,其中四環(huán)素類tet L基因的檢出率高達(dá)92.39%,多黏菌素類mcr-1基因及大環(huán)內(nèi)酯類erm B基因的檢出率分別為79.35%和76.09%。對共同攜帶的耐藥基因進(jìn)行同源性分析表明,耐藥基因tet L和erm B的同源性較高,均達(dá)到97%以上,其中tet L基因達(dá)到100%。研究結(jié)果提示豬場豬源和人源鏈球菌對多種常用獸用抗菌藥及人用抗菌藥物產(chǎn)生共同表觀耐藥性,而其部分相關(guān)耐藥基因具有高度的同源性,這表明細(xì)菌耐藥性及其耐藥基因可在豬源與人源鏈球菌中傳播。
[Abstract]:The sensitivity tests of 11 veterinary drugs and 11 human antimicrobial agents were carried out by microdilution method. The results showed that the pig source and human Streptococcus mutans produced apparent resistance to 8 kinds of veterinary drugs and 10 kinds of human antibiotics. The resistance genes were detected for the isolated strains and 3 kinds of antibiotics with high common resistance rates were selected. 9 major related genes were detected, and 6 kinds of common resistance genes were found in pig and human Streptococcus, of which the detection rate of tetracycline Tet L was up to 92.39%. The detection rates of polymyxin mcr-1 gene and macrolide ERM B gene were respectively 79.35% and 76.09%. to carry out homologous analysis of common resistant genes. The results showed that the homology of Tet L and ERM B was higher than 97%. The Tet L gene reached the results of 100%., which suggested that pig farm and Streptococcus human produced common apparent resistance to a variety of common veterinary drugs and human antimicrobial agents, and that some of their related drug resistance genes were highly homologous, which indicated that bacteria were resistant to bacteria. Drug resistance and drug resistance genes can be transmitted from swine to human Streptococcus.

【作者單位】： 錦州醫(yī)科大學(xué)畜牧獸醫(yī)學(xué)院;遼寧省獸藥飼料畜產(chǎn)品質(zhì)量安全檢測中心;遼寧省錦州市動(dòng)物檢疫站;遼寧省本溪市動(dòng)物疫病預(yù)防控制中心;
【基金】：遼寧省科技廳自然科學(xué)基金項(xiàng)目(2013022047) 國家自然科學(xué)基金(31201951)
【分類號(hào)】：S852.61

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