本文選題：小菜蛾 + β-��； 參考：《福建師范大學(xué)學(xué)報(bào)(自然科學(xué)版)》2017年05期

【摘要】：為研究小菜蛾的先天免疫機(jī)制,從小菜蛾幼蟲體內(nèi)克隆得到一條β-1,3-葡聚糖識別蛋白(β-1,3-glucan recognition protein,βGRP)基因,利用生物信息學(xué)軟件對該基因及其編碼蛋白的理化性質(zhì)、疏水性、結(jié)構(gòu)等方面進(jìn)行分析與預(yù)測,同時(shí)構(gòu)建系統(tǒng)進(jìn)化樹.為研究該蛋白的功能,以大腸桿菌為宿主表達(dá)該基因,利用鎳柱純化目的蛋白.實(shí)驗(yàn)結(jié)果表明,該序列的開放閱讀框長1 287 bp,編碼428個(gè)氨基酸,編碼的蛋白質(zhì)理論分子質(zhì)量約48.13 ku,理論的等電點(diǎn)為6.18,為親水性蛋白;進(jìn)化樹分析表明,該蛋白跟家蠶、柞蠶、菜青蟲、棉鈴蟲、冬尺蠖蛾、粉紋夜蝶歸為同一大類;經(jīng)大腸桿菌表達(dá)的蛋白均為不溶性的包涵體,通過對包涵體的變性及鎳柱的純化,得到了濃度較高的可溶性目的蛋白.
[Abstract]:In order to study the innate immune mechanism of the Plutella xylostella, a beta -1,3- glucan identification protein (beta -1,3-glucan recognition protein, beta GRP) gene was cloned from the larvae of the Plutella xylostella, and the bioinformatics software was used to analyze and predict the physicochemical properties, hydrophobicity and structure of the gene and its encoded protein. At the same time, the system was constructed. In order to study the function of the protein, the gene was expressed in Escherichia coli and the target protein was purified with a nickel column. The experimental results showed that the open reading frame of the sequence was 1287 BP long, 428 amino acids encoded, the theoretical molecular weight of the encoded protein was about 48.13 Ku, the theoretical isoelectric point was 6.18, the hydrophilic protein, and the phylogenetic tree analysis table. The protein is classified as the same big class with silkworm, tussah, vegetable, cotton bollworm, cotton bollworm, worm moth, and Silverworm. The proteins expressed in Escherichia coli are insoluble inclusion bodies. By denaturation of inclusion bodies and purification of nickel columns, a soluble target protein with high concentration is obtained.

【作者單位】： 福建師范大學(xué)生命科學(xué)學(xué)院;
【基金】：福建省教育廳資助項(xiàng)目(JK2012009)
【分類號】：S433.4
，

本文編號：1881963