本文選題：陸地棉 + GhHAK��； 參考：《中國農(nóng)業(yè)科技導(dǎo)報》2017年10期

【摘要】：棉花是一種典型的喜鉀經(jīng)濟作物,其產(chǎn)量和品質(zhì)形成與鉀含量密切相關(guān)�？寺～@得棉花高親和性鉀轉(zhuǎn)運體基因GhHAK1框,系統(tǒng)進(jìn)化樹表明該GhHAK1基因?qū)儆诘冖駚喿?此分支被認(rèn)為介導(dǎo)高親和性K+。膜結(jié)構(gòu)預(yù)測顯示該基因編碼的蛋白跨膜螺旋區(qū)域(TMS)有12個,且在第2和第3 TMS之間有一個較長的胞質(zhì)質(zhì)環(huán)。洋蔥表皮亞細(xì)胞定位表明,該基因編碼蛋白定位在細(xì)胞質(zhì)膜上;繼而對部分轉(zhuǎn)GhHAK1基因擬南芥純合系進(jìn)行K+營養(yǎng)試驗,發(fā)現(xiàn)在低鉀(0.05 mmol/L)條件下,野生型擬南芥表現(xiàn)明顯的缺鉀癥狀,葉片黃化,植株抽薹明顯受抑制,而轉(zhuǎn)基因材料則表現(xiàn)植株葉片生長正常,抽薹未受影響,同時轉(zhuǎn)基因擬南芥葉片中K+含量約為野生型擬南芥的2倍左右,根中K+含量約為1.5倍左右。該結(jié)果初步表明GhHAK1基因具有介導(dǎo)植株鉀高效吸收的功能,為進(jìn)一步培育適應(yīng)土壤鉀素匱乏及鹽漬化環(huán)境下生長的棉花新品種提供重要的基因資源。
[Abstract]:Cotton is a typical potassium-loving cash crop, and its yield and quality are closely related to potassium content. The GhHAK1 frame of high affinity potassium transporter gene in cotton was cloned. The phylogenetic tree showed that the GhHAK1 gene belonged to the first subfamily, and this branch was considered to mediate high affinity K. Membrane structure prediction showed that there were 12 transmembrane helical regions of the protein encoded by the gene, and a long cytoplasmic ring existed between the second and third TMS. Onion epidermis subcellular localization showed that the gene encoded protein was located on the cytoplasmic membrane, and then K nutrition tests were carried out on homozygous lines of Arabidopsis thaliana partially transformed with GhHAK1 gene, and it was found that under the condition of low potassium (0. 05 mmol / L), Wild-type Arabidopsis thaliana showed obvious symptoms of potassium deficiency, leaf yellowing, bolting inhibition, while transgenic plants showed normal leaf growth and unaffected bolting. The K content of transgenic Arabidopsis thaliana leaves was about 2 times as high as that of wild type Arabidopsis thaliana, and the K content in roots was about 1.5 times higher than that of wild type Arabidopsis thaliana. The results indicated that the GhHAK1 gene had the function of mediating the high efficiency absorption of potassium in plants, which provided important genetic resources for further cultivation of cotton varieties adapted to soil potassium deficiency and salinization.
【作者單位】： 河北大學(xué)生命科學(xué)學(xué)院;河北省承德市興隆縣農(nóng)牧局;河北大學(xué)經(jīng)濟學(xué)院;
【基金】：河北省自然科學(xué)基金項目(C2015201227) 河北大學(xué)人才引進(jìn)項目(2014-277)資助
【分類號】：Q943.2;S562
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本文編號：1880189