發(fā)布時(shí)間：2018-05-11 03:42

  本文選題：葡萄 + GAMYB　； 參考：《園藝學(xué)報(bào)》2017年06期

【摘要】：以‘魏可’葡萄(Vitis vinifera L.‘Wink’)為試材,克隆得到一個(gè)葡萄赤霉素信號(hào)途徑中的MYB轉(zhuǎn)錄因子基因Vv GAMYB。該基因開(kāi)放閱讀框(ORF)長(zhǎng)1 680 bp,編碼559個(gè)氨基酸,該蛋白序列含有GAMYB基因家族保守的R2R3 DNA結(jié)合域以及Box1、Box2、Box3保守域。洋蔥表皮細(xì)胞的瞬時(shí)表達(dá)結(jié)果顯示,VvGAMYB定位于細(xì)胞核中。VvGAMYB和其下游基因Vv LFY在葡萄花發(fā)育過(guò)程中的表達(dá)都呈現(xiàn)先上升后下降的趨勢(shì),而赤霉素處理促進(jìn)了VvGAMYB和VvLFY的表達(dá),從而使開(kāi)花提前。VvGAMYB是miR159的靶基因,其作用受到miR159的調(diào)控。miR159和VvGAMYB的表達(dá)負(fù)相關(guān),赤霉素處理下調(diào)miR159表達(dá)從而也降低了對(duì)VvGAMYB的裂解作用。由上述結(jié)果可以看出,VvGAMYB通過(guò)赤霉素開(kāi)花途徑參與葡萄的開(kāi)花過(guò)程。在這一過(guò)程中,赤霉素抑制miR159裂解VvGAMYB的作用,從而上調(diào)VvLFY的基因表達(dá),參與了調(diào)控葡萄開(kāi)花過(guò)程。
[Abstract]:A MYB transcription factor gene Vv GAMYB in the gibberellin signaling pathway was cloned from 'Vitis vinifera L.wink'. The ORF gene is 1680bplong and encodes 559 amino acids. The protein sequence contains the conserved R2R3 DNA binding domain of GAMYB gene family and the conserved domain of Box1 gene family Box2 and Box3. The transient expression of VvGAMYB in the epidermis of onion showed that the expression of VvGAMYB and VvGAMYB and its downstream gene Vv LFY increased first and then decreased during the development of grape flower, while gibberellin treatment promoted the expression of VvGAMYB and VvLFY. VvGAMYB was the target gene of miR159, and its effect was negatively correlated with the expression of miR159. MiR159 and VvGAMYB. Gibberellin treatment could down-regulate the expression of miR159 and thus reduce the lytic effect of VvGAMYB. From the above results, it can be seen that VvGAMYB is involved in the flowering process of grape through gibberellin flowering pathway. In this process, gibberellin inhibits the cleavage of VvGAMYB by miR159, thus upregulating the gene expression of VvLFY, which is involved in the regulation of grape flowering.
【作者單位】： 南京農(nóng)業(yè)大學(xué)園藝學(xué)院;江蘇農(nóng)林職業(yè)技術(shù)學(xué)院;
【基金】：中國(guó)博士后科學(xué)基金項(xiàng)目(802602) 江蘇省博士后科研資助計(jì)劃項(xiàng)目(82161015)
【分類號(hào)】：S663.1
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本文編號(hào)：1872282