發(fā)布時間：2018-05-11 03:42

  本文選題：葡萄 + GAMYB　； 參考：《園藝學報》2017年06期

【摘要】：以‘魏可’葡萄(Vitis vinifera L.‘Wink’)為試材,克隆得到一個葡萄赤霉素信號途徑中的MYB轉(zhuǎn)錄因子基因Vv GAMYB。該基因開放閱讀框(ORF)長1 680 bp,編碼559個氨基酸,該蛋白序列含有GAMYB基因家族保守的R2R3 DNA結(jié)合域以及Box1、Box2、Box3保守域。洋蔥表皮細胞的瞬時表達結(jié)果顯示,VvGAMYB定位于細胞核中。VvGAMYB和其下游基因Vv LFY在葡萄花發(fā)育過程中的表達都呈現(xiàn)先上升后下降的趨勢,而赤霉素處理促進了VvGAMYB和VvLFY的表達,從而使開花提前。VvGAMYB是miR159的靶基因,其作用受到miR159的調(diào)控。miR159和VvGAMYB的表達負相關(guān),赤霉素處理下調(diào)miR159表達從而也降低了對VvGAMYB的裂解作用。由上述結(jié)果可以看出,VvGAMYB通過赤霉素開花途徑參與葡萄的開花過程。在這一過程中,赤霉素抑制miR159裂解VvGAMYB的作用,從而上調(diào)VvLFY的基因表達,參與了調(diào)控葡萄開花過程。
[Abstract]:A MYB transcription factor gene Vv GAMYB in the gibberellin signaling pathway was cloned from 'Vitis vinifera L.wink'. The ORF gene is 1680bplong and encodes 559 amino acids. The protein sequence contains the conserved R2R3 DNA binding domain of GAMYB gene family and the conserved domain of Box1 gene family Box2 and Box3. The transient expression of VvGAMYB in the epidermis of onion showed that the expression of VvGAMYB and VvGAMYB and its downstream gene Vv LFY increased first and then decreased during the development of grape flower, while gibberellin treatment promoted the expression of VvGAMYB and VvLFY. VvGAMYB was the target gene of miR159, and its effect was negatively correlated with the expression of miR159. MiR159 and VvGAMYB. Gibberellin treatment could down-regulate the expression of miR159 and thus reduce the lytic effect of VvGAMYB. From the above results, it can be seen that VvGAMYB is involved in the flowering process of grape through gibberellin flowering pathway. In this process, gibberellin inhibits the cleavage of VvGAMYB by miR159, thus upregulating the gene expression of VvLFY, which is involved in the regulation of grape flowering.
【作者單位】： 南京農(nóng)業(yè)大學園藝學院;江蘇農(nóng)林職業(yè)技術(shù)學院;
【基金】：中國博士后科學基金項目(802602) 江蘇省博士后科研資助計劃項目(82161015)
【分類號】：S663.1
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本文編號：1872282