本文選題：烏珠穆沁羊 + 肌肉生長抑制素��； 參考：《中國食品學報》2017年04期

【摘要】：采用純種(多胸椎個體)烏珠穆沁羊,對該品種MSTN基因進行PCR分段擴增并克隆和測序。利用DNAMAN進行序列拼接,獲得烏珠穆沁羊MSTN基因全長序列。該基因由3個外顯子和2個內含子組成,外顯子大小分別為373,374,381 bp,內含子大小分別為1 833,2 030 bp。CDS序列全長為1 128 bp,由375個氨基酸組成。MSTN基因編碼蛋白的理論分子質量約為42.8 ku,PI值為7.01,亮氨酸的含量最高(9.9%),其次是賴氨酸(8.3%)。烏珠穆沁羊基因編碼蛋白二級結構以隨機卷曲結構為主,屬于跨膜蛋白,跨膜區(qū)位于AA6-AA23之間,具有一個分泌信號肽結構。其氨基酸序列MQKLQICVYIYLFMLIVA具有TGF-β家族的特征。
[Abstract]:The MSTN gene of this breed was amplified by PCR, cloned and sequenced. The full-length sequence of MSTN gene was obtained by using DNAMAN sequence splicing. The gene consists of three exons and two introns. The exon size is 373374381 BP, the intron size is 1 833 bp.CDS and the total length is 1 128 BP. The theoretical molecular weight of the protein encoded by the 375-amino acid gene is about 42.8kuN Pi value 7.01, the highest content of leucine is 9.9B, and the second is lysine 8.3bp. The secondary structure of the protein encoded by Uzhimuqin sheep gene is mainly random curl structure, which belongs to transmembrane protein. The transmembrane region is located between AA6-AA23 and has a secretory signal peptide structure. Its amino acid sequence MQKLQICVYIYLFMLIVA has the characteristic of TGF- 尾 family.
【作者單位】： 內蒙古農業(yè)大學食品科學與工程學院;內蒙古錫林浩特市農牧業(yè)局;
【基金】：國家自然科學基金項目(31360368) 內蒙古自然科學基金項目(2015MS0361) 內蒙古自治區(qū)組織部內蒙古草原英才基金
【分類號】：Q953

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