本文選題：棉花 + MYB轉(zhuǎn)錄因子�。� 參考：《作物學(xué)報(bào)》2016年09期

【摘要】：MYB轉(zhuǎn)錄因子是在真核生物細(xì)胞內(nèi)廣泛存在的一類轉(zhuǎn)錄因子蛋白,在植物的生長(zhǎng)發(fā)育、代謝調(diào)節(jié)、抗病抗逆、以及激素介導(dǎo)的信號(hào)路徑等方面都發(fā)揮著重要的作用。本研究從海島棉品種海7124中克隆得到一個(gè)MYB轉(zhuǎn)錄因子基因,根據(jù)序列同源性和進(jìn)化分析,將其命名為GbMYB60。該基因序列長(zhǎng)990 bp,編碼一個(gè)36.9 k D的R2R3類MYB轉(zhuǎn)錄因子蛋白。GbMYB60蛋白被特異地定位于植物細(xì)胞核。GbMYB60基因的表達(dá)水平較低,在真葉中優(yōu)勢(shì)表達(dá),根系中表達(dá)量最低。該基因受甘露醇、Na Cl、低溫、高溫等非生物逆境,以及脫落酸、乙烯利、茉莉酸甲酯和水楊酸等植物激素的誘導(dǎo)上調(diào)表達(dá)。利用病毒誘導(dǎo)的基因沉默技術(shù)在海島棉中干涉GbMYB60發(fā)現(xiàn),降低GbMYB60基因的表達(dá)水平,使棉花幼苗對(duì)高鹽脅迫的耐受性降低;但GbMYB60干涉的植株在甘露醇溶液的處理下與對(duì)照植株并無顯著的抗性差異。
[Abstract]:MYB transcription factors are a class of transcription factor proteins widely existing in eukaryotic cells, which play an important role in plant growth and development, metabolic regulation, disease resistance and stress resistance, as well as hormone mediated signal pathway. A MYB transcription factor gene was cloned from sea island cotton variety Hai 7124 and named GbMYB60 according to sequence homology and evolution analysis. The length of the gene was 990 BP, encoding a 36.9 KD R2R3 like transcription factor protein. GbMYB60 protein was specifically located in the plant nucleus. The expression level of GbMYB60 gene was lower than that in the true leaves, and the lowest in the root system. The gene was up-regulated by abiotic stresses such as mannitol, low temperature, high temperature, and plant hormones such as abscisic acid, ethephon, methyl jasmonate and salicylic acid. The technique of virus-induced gene silencing was used to interfere with GbMYB60 in island cotton. It was found that the expression level of GbMYB60 gene was decreased and the tolerance of cotton seedlings to high salt stress was decreased. However, there was no significant difference in resistance between the plants treated with mannitol and the control plants treated with GbMYB60 interference.
【作者單位】： 河南大學(xué)生命科學(xué)學(xué)院/棉花生物學(xué)國家重點(diǎn)實(shí)驗(yàn)室/植物逆境生物學(xué)重點(diǎn)實(shí)驗(yàn)室;
【基金】：國家重點(diǎn)基礎(chǔ)研究發(fā)展計(jì)劃項(xiàng)目(2012CB1143001) 棉花生物學(xué)國家重點(diǎn)實(shí)驗(yàn)室開放課題(CB2015A31) 河南省教育廳項(xiàng)目(15A180028,15A180029)資助~~
【分類號(hào)】：S562
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本文編號(hào)：1857662