本文選題：陸地棉 + 抗凋亡基因DAD��； 參考：《植物生理學(xué)報(bào)》2017年08期

【摘要】：抗凋亡基因DAD1是細(xì)胞程序性死亡的抑制基因,在植物生長(zhǎng)發(fā)育中發(fā)揮重要作用。為探討該基因在具有AADD基因組的異源四倍體陸地棉馴化過程中的遺傳改變,本研究選取了陸地棉的野生和栽培樣品作為研究對(duì)象,結(jié)合分子克隆、測(cè)序以及棉纖維不同發(fā)育階段的轉(zhuǎn)錄組數(shù)據(jù)分析等方法,對(duì)二者在DAD1基因序列和表達(dá)水平的差異進(jìn)行了深入比較,結(jié)果顯示:陸地棉中,DAD1基因同時(shí)具有A和D基因組的2個(gè)同源拷貝;馴化對(duì)這2個(gè)拷貝的影響是不均衡的,其中A基因組的拷貝在基因序列上保持一致,但在表達(dá)上出現(xiàn)分化;D基因組拷貝在基因序列上出現(xiàn)了5個(gè)核苷酸的變異,包括1個(gè)發(fā)生在外顯子區(qū)的同義突變,但在表達(dá)上卻未出現(xiàn)明顯變化。我們目前的研究結(jié)果展示了馴化對(duì)多倍體植物重要功能基因同源組分的影響是獨(dú)立的,非對(duì)稱性的。
[Abstract]:Antiapoptotic gene DAD1 is an inhibitor of programmed cell death and plays an important role in plant growth and development. In order to investigate the genetic changes of the gene during acclimation of allotetraploid upland cotton with AADD genome, the wild and cultivated samples of upland cotton were selected as the research objects, and the molecular cloning was combined. Sequencing and transcriptome data analysis of cotton fiber at different developmental stages were used to compare the difference of DAD1 gene sequence and expression level between them. The results showed that there were two homologous copies of A and D genomes in upland cotton, and the effects of acclimation on the two copies were uneven, and the copies of A genome were consistent in gene sequence. However, there were five nucleotide variations in the gene sequence, including one synonymous mutation in exon region, but no significant change in expression. Our results show that the effects of acclimation on the autofractions of important functional genes in polyploid plants are independent and asymmetric.
【作者單位】： 曲阜師范大學(xué)生命科學(xué)學(xué)院;
【基金】：國(guó)家自然科學(xué)基金(31570218)~~
【分類號(hào)】：S562
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本文編號(hào)：1853286