本文選題：陸地棉 + 抗凋亡基因DAD��； 參考：《植物生理學報》2017年08期

【摘要】：抗凋亡基因DAD1是細胞程序性死亡的抑制基因,在植物生長發(fā)育中發(fā)揮重要作用。為探討該基因在具有AADD基因組的異源四倍體陸地棉馴化過程中的遺傳改變,本研究選取了陸地棉的野生和栽培樣品作為研究對象,結合分子克隆、測序以及棉纖維不同發(fā)育階段的轉錄組數(shù)據分析等方法,對二者在DAD1基因序列和表達水平的差異進行了深入比較,結果顯示:陸地棉中,DAD1基因同時具有A和D基因組的2個同源拷貝;馴化對這2個拷貝的影響是不均衡的,其中A基因組的拷貝在基因序列上保持一致,但在表達上出現(xiàn)分化;D基因組拷貝在基因序列上出現(xiàn)了5個核苷酸的變異,包括1個發(fā)生在外顯子區(qū)的同義突變,但在表達上卻未出現(xiàn)明顯變化。我們目前的研究結果展示了馴化對多倍體植物重要功能基因同源組分的影響是獨立的,非對稱性的。
[Abstract]:Antiapoptotic gene DAD1 is an inhibitor of programmed cell death and plays an important role in plant growth and development. In order to investigate the genetic changes of the gene during acclimation of allotetraploid upland cotton with AADD genome, the wild and cultivated samples of upland cotton were selected as the research objects, and the molecular cloning was combined. Sequencing and transcriptome data analysis of cotton fiber at different developmental stages were used to compare the difference of DAD1 gene sequence and expression level between them. The results showed that there were two homologous copies of A and D genomes in upland cotton, and the effects of acclimation on the two copies were uneven, and the copies of A genome were consistent in gene sequence. However, there were five nucleotide variations in the gene sequence, including one synonymous mutation in exon region, but no significant change in expression. Our results show that the effects of acclimation on the autofractions of important functional genes in polyploid plants are independent and asymmetric.
【作者單位】： 曲阜師范大學生命科學學院;
【基金】：國家自然科學基金(31570218)~~
【分類號】：S562
，

本文編號：1853286