本文選題：心肌梗死 + miR--p��； 參考：《實(shí)用醫(yī)學(xué)雜志》2016年22期

【摘要】：目的:探討miR-455-3p在心肌梗死后心肌肥厚中的作用。方法:比較miR-455-3p在心肌梗死組和空白對(duì)照組的表達(dá)。心肌細(xì)胞過表達(dá)miR-455-3p情況下分析細(xì)胞表面積和心肌肥厚基因表達(dá)。熒光素酶活性檢測(cè)和Western blot法確定受miR-455-3p調(diào)控的靶基因。通過心肌梗死模型組大鼠體內(nèi)miR-455-3p的過表達(dá)確定miR-455-3p對(duì)心肌肥厚的影響。結(jié)果:在體外和體內(nèi)試驗(yàn)中發(fā)現(xiàn)在心肌肥厚中miR-455-3p均被下調(diào)。體外實(shí)驗(yàn),過度表達(dá)miR-455-3p可以抑制心肌肥厚。與miR-455-3p相比,心肌肥厚中HDAC2被上調(diào)。HDAC2是一種新型的miR-455-3p靶基因。心肌梗死大鼠過表達(dá)miR-455-3p通過降低HDAC2的表達(dá)能抑制心肌梗死后心肌肥厚。結(jié)論:miR-455-3p是心肌肥厚的重要調(diào)節(jié)因子,為心肌梗死后心肌肥厚的治療提供新的理論基礎(chǔ)。
[Abstract]:Objective: to investigate the role of miR-455-3p in myocardial hypertrophy after myocardial infarction. Methods: to compare the expression of miR-455-3p in myocardial infarction group and blank control group. Cell surface area and myocardial hypertrophy gene expression were analyzed when myocardial cells were overexpressed by miR-455-3p. Luciferase activity and Western blot assay were used to identify target genes regulated by miR-455-3p. The effect of miR-455-3p on myocardial hypertrophy was determined by overexpression of miR-455-3p in myocardial infarction model group. Results: miR-455-3p was down-regulated in myocardial hypertrophy in vitro and in vivo. In vitro, overexpression of miR-455-3p can inhibit myocardial hypertrophy. Compared with miR-455-3p, HDAC2 is up-regulated in myocardial hypertrophy. HDAC2 is a novel miR-455-3p target gene. Overexpression of miR-455-3p can inhibit myocardial hypertrophy after myocardial infarction by decreasing the expression of HDAC2. Conclusion the ratio miR-455-3p is an important regulatory factor for myocardial hypertrophy, which provides a new theoretical basis for the treatment of myocardial hypertrophy after myocardial infarction.
【作者單位】： 鄭州大學(xué)第二附屬醫(yī)院心內(nèi)科;鄭州大學(xué)第二附屬醫(yī)院干部病房;
【基金】：河南省科技攻關(guān)計(jì)劃項(xiàng)目(編號(hào):142102310109) 河南省高等學(xué)校重點(diǎn)科研項(xiàng)目(編號(hào):15A320019)
【分類號(hào)】：R542.22
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本文編號(hào)：1853053