本文選題：蜻蜓鳳梨 + AP/EREBP家族��； 參考：《西北農(nóng)業(yè)學報》2016年12期

【摘要】：觀賞鳳梨是一類重要的熱帶花卉,但有關(guān)其生長發(fā)育調(diào)控分子機理的研究相對匱乏,這在一定程度上限制了新品種的開發(fā)和利用。以熱帶觀賞花卉蜻蜓鳳梨(Aechemia fasciata)為材料,在分析蜻蜓鳳梨轉(zhuǎn)錄組數(shù)據(jù)的基礎(chǔ)上,結(jié)合cDNA末端快速擴增(rapid amplification of cDNA ends,RACE)技術(shù)克隆了APETALA2/Ethylene Response Element Binding Protein(AP2/EREBP)家族的1個轉(zhuǎn)錄因子編碼序列,并將其命名為AfERF113。生物信息學分析表明,AfERF113 cDNA全長1 093bp,有1個864bp的開放閱讀框(open reading frame,ORF),編碼287個氨基酸。蛋白質(zhì)理化性質(zhì)分析表明,該蛋白分子質(zhì)量為29.768 5ku,理論等電點為6.06,為一類不穩(wěn)定的酸性親水蛋白。亞細胞定位軟件預(yù)測表明該蛋白定位于細胞核。結(jié)構(gòu)域分析表明,該蛋白有1個保守的AP2結(jié)構(gòu)域,分屬ERF亞族的B-4類,與擬南芥中所有ERF蛋白的ERF113親源關(guān)系最近。實時熒光定量PCR(quantitative real time PCR,qRT-PCR)結(jié)果表明,AfERF113轉(zhuǎn)錄本在成熟株的根中表達量最高,且外源乙烯誘導后,AfERF113的表達量無論是在幼株還是在成株的各組織中均顯著上調(diào)。研究結(jié)果證實,AfERF113響應(yīng)了外源乙烯調(diào)控,為進一步研究AfERF113基因的功能及通過基因工程手段調(diào)控鳳梨科植物生長發(fā)育提供了理論依據(jù)。
[Abstract]:Ornamental pineapple is an important tropical flower, but the molecular mechanism of its growth and development regulation is relatively scarce, which limits the development and utilization of new varieties to a certain extent. Using Aechemia fasciata, a tropical ornamental flower, as a material, based on the analysis of transcriptional data, a transcription factor encoding sequence of APETALA2/Ethylene Response Element Binding protein AP2 / EREBP family was cloned by cDNA terminal rapid amplification of cDNA endsracea technique. And named Aferf113. Bioinformatics analysis showed that the cDNA of AfERF113 was 1 093bp. there was an open reading frame of 864bp, which encodes 287-amino acid. The physicochemical properties of the protein show that the molecular weight of the protein is 29.768 kuand the theoretical isoelectric point is 6.06. it is a kind of unstable acidic hydrophilic protein. Subcellular localization software predicted that the protein was located in the nucleus. Domain analysis showed that the protein had a conserved AP2 domain, belonging to B-4 of ERF subfamily, and had the closest relationship with ERF113 of all ERF proteins in Arabidopsis. The results of real-time PCR(quantitative real time PCR qRT-PCRR showed that the expression of AfERF113 transcripts was the highest in the roots of mature strains, and the expression of AfERF113 was upregulated in both young and adult tissues after exogenous ethylene induction. The results showed that AfERF113 responded to exogenous ethylene regulation, which provided a theoretical basis for further study on the function of AfERF113 gene and regulation of the growth and development of pineapple plants by means of genetic engineering.
【作者單位】： 中國熱帶農(nóng)業(yè)科學院熱帶作物品種資源研究所農(nóng)業(yè)部華南作物基因資源與種質(zhì)創(chuàng)新重點實驗室;
【基金】：國家自然基金面上項目(31372106) 海南省自然科學基金面上項目(20163127)~~
【分類號】：S682.3

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