本文選題：枳橙 + 硝態(tài)氮　； 參考：《果樹學(xué)報》2017年04期

【摘要】：【目的】進一步探討枳橙對氮素吸收利用機制,為柑橘生產(chǎn)上提高氮素利用效率和科學(xué)施肥提供科學(xué)依據(jù)�！痉椒ā恳澡壮葹椴牧�,采用水培方法,在Hoagland配方的基礎(chǔ)上進行調(diào)節(jié),研究了不同形態(tài)氮素配比對枳橙幼苗硝酸還原酶(NR)活性變化及相關(guān)基因表達量的影響�！窘Y(jié)果】幼苗葉片及根系NR活性均隨著培養(yǎng)時間的延長而增加,且葉片NR活性高于根系。其中2號(NO_3~-∶NH_4~+=7∶3)和3號(NO_3~-∶NH_4~+=5∶5)處理的葉片酶活最高;1號(NO_3~-∶NH_4~+=10∶0)和2號(NO_3~-∶NH_4~+=7∶3)處理根系的NR活性顯著高于其他處理;進一步研究NR相關(guān)基因表達情況表明:3個基因(CitNia1、CitNia2、CitNia OA)在不同處理的枳橙幼苗體內(nèi)均有表達,其中CitNia1和CitNia2分別是根系和葉片NR相關(guān)主效基因,2者均受到NO_3~-、NH_4~+配比誘導(dǎo),且3號處理(NO_3~-∶NH_4~+=5∶5)下表達量最高�！窘Y(jié)論】枳橙幼苗硝態(tài)氮代謝的主要部位為葉片,且CitNia2是主效基因,通過調(diào)節(jié)銨硝比例可以促進其表達。
[Abstract]:[objective] to further explore the mechanism of nitrogen absorption and utilization in citrange, so as to provide scientific basis for improving nitrogen use efficiency and scientific fertilization in citrus production. Based on the Hoagland formula, The effects of different forms of nitrogen on nitrate reductase (NR) activity and the expression of related genes were studied. [results] the NR activity of leaves and roots increased with the increase of culture time, and the NR activity of leaves was higher than that of roots. The highest enzyme activity was found in the leaves treated by the two kinds of treatments, No3 (1) and 10: 10 (10: 10: 10: 10: 0) and No3 (No3) and (3) No3 / S / NH4 ~ 5: 5), respectively. The NR activity of the roots of the two treatments was significantly higher than that of the other treatments in the treatment of No3 + 10: 10: 10: 0) and in the treatment of No.2 / No3 / S / VNH4 ~ 7w / 3), respectively, in which the activity of NR was significantly higher than that of the other treatments. Further study on the expression of NR related genes showed that CitNia1 CitNia2 CitNia OAA was expressed in different treatments of CitNia2, and CitNia1 and CitNia2 were induced by NO3 + -NH4- + ratio in roots and leaves, respectively. The expression of nitrate nitrogen in trifoliate orange seedlings was the highest under the condition of No3 +-10% NH _ 4 ~ 5: 5. [conclusion] the main part of nitrate nitrogen metabolism in trifoliate orange seedlings was leaf, and CitNia2 was a major gene, which could be promoted by adjusting the ratio of ammonium nitrate.
【作者單位】： 湖南農(nóng)業(yè)大學(xué)園藝園林學(xué)院;中南林業(yè)科技大學(xué)林學(xué)院;
【基金】：現(xiàn)代農(nóng)業(yè)(柑橘)產(chǎn)業(yè)技術(shù)體系(CARS-027) 國家自然科學(xué)基金(31401824) 教育部高校博士點基金(20134320120006)
【分類號】：S666.4

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