本文選題：馬鈴薯 + 耐寒性��； 參考：《湖南農(nóng)業(yè)大學(xué)》2016年碩士論文

【摘要】：我國馬鈴薯一年四季均有種植,種植地域廣泛。生產(chǎn)中馬鈴薯常遭受低溫脅迫的危害,特別是對南方冬種馬鈴薯影響較大。該論文分別以主要馬鈴薯栽培品種大西洋和東農(nóng)303為對照,以導(dǎo)入亞精胺合成酶基因(SPDS)的01-6、01-47、01-49、和02-42、02-57、02-72株系為供試材料,研究了轉(zhuǎn)SPDS馬鈴薯植株農(nóng)藝性狀表現(xiàn)、光合活性變化、耐寒性的變化,以及供試材料生理指標(biāo)變化。主要結(jié)果如下：1轉(zhuǎn)SPDS基因馬鈴薯農(nóng)藝性狀評價通過觀測轉(zhuǎn)SPDS基因馬鈴薯植株的田間性狀得出,所有供試的轉(zhuǎn)SPDS基因馬鈴薯植株在株高、莖粗、葉片數(shù)(片)、枝節(jié)數(shù)(個)等方面較優(yōu)于對照馬鈴薯植株。通過對轉(zhuǎn)SPDS基因馬鈴薯進(jìn)行主要品質(zhì)成分分析得出,01-6、01-47和02-42的主要品質(zhì)成分與對照馬鈴薯無明顯差異。2低溫脅迫對轉(zhuǎn)SPDS基因馬鈴薯植株光合活性的影響通過測定低溫脅迫期間轉(zhuǎn)SPDS基因馬鈴薯植株的葉綠素含量、凈光合速率、氣孔導(dǎo)度、胞間CO2濃度和蒸騰速率可知：01-47、01-49、02-42、02-57和02-72的葉綠素含量均高于對照馬鈴薯植株。轉(zhuǎn)SPDS基因植株的凈光合速率高于對照馬鈴薯植株,轉(zhuǎn)SPDS基因馬鈴薯植株的氣孔導(dǎo)度大于對照馬鈴薯植株,轉(zhuǎn)SPDS基因馬鈴薯植株的胞間CO2濃度低于對照馬鈴薯植株,轉(zhuǎn)SPDS基因馬鈴薯植株的蒸騰速率高于未對照馬鈴薯植株�？梢哉f明：轉(zhuǎn)SPDS基因馬鈴薯植株的光合活性強(qiáng)于對照馬鈴薯植株。3轉(zhuǎn)SPDS馬鈴薯植株耐寒性的檢測通過測定在不同低溫下轉(zhuǎn)SPDS基因馬鈴薯植株的離體葉片的電導(dǎo)率可知,轉(zhuǎn)SPDS基因馬鈴薯在低溫脅迫下的相對電導(dǎo)率上升較對照馬鈴薯緩慢,01-6、01-47和01-49在-4℃相對電導(dǎo)率超過50%,較對照低了1℃。02-42、02-57在-4℃時相對電導(dǎo)率超過50%,較對照低了1℃。由此分析轉(zhuǎn)SPDS馬鈴薯植株的半致死溫度LT50得出,轉(zhuǎn)SPDS基因馬鈴薯的半致死溫度均低于對照馬鈴薯植株,其耐寒性優(yōu)于對照。4低溫脅迫對轉(zhuǎn)SPDS基因馬鈴薯植株生理指標(biāo)的影響通過測定低溫脅迫期間轉(zhuǎn)SPDS基因馬鈴薯植株丙二醛(MDA)的含量、游離脯氨酸含量、過氧化物酶(POD)活性和超氧化物歧化酶(SOD)的活性的變化得出：轉(zhuǎn)SPDS基因馬鈴薯01-49和02-42的丙二醛(MDA)含量在低溫脅迫下低于對照馬鈴薯植株。轉(zhuǎn)SPDS基因馬鈴薯01-47、01-49、02-42和02-57的游離脯氨酸含量在低溫脅迫期間高于對照馬鈴薯植株。所有供試的轉(zhuǎn)SPDS基因馬鈴薯的過化物歧化酶(POD)活性在低溫脅迫期間高于對照馬鈴薯植株。轉(zhuǎn)SPDS馬鈴薯01-6、01-47、02-42和02-57的超氧化物歧化酶(SOD)活性在低溫脅迫期間高于對照馬鈴薯植株。
[Abstract]:Potato is planted all year round in our country, and the planting area is wide. Potato is often damaged by low temperature stress, especially the southern winter potato. In this paper, the main potato cultivar Atlantic and Dongnong 303 were used as control, and the lines 01-6C01-47DS-01-49and 02-42C02-57G02-72 introduced spermidine synthase gene (SPDS), respectively, were used to study the agronomic characters and the changes of photosynthetic activity of potato plants transformed with SPDS. The changes of cold tolerance and physiological indexes of the tested materials. The main results were as follows: 1. The agronomic characters of potato transformed with SPDS gene were evaluated. By observing the field characters of potato plants with SPDS gene, the results showed that all the transgenic potato plants with SPDS gene were at plant height and stem diameter. The number of leaves and branches were better than that of the control potato. By analyzing the main quality components of transgenic potato with SPDS gene, it is concluded that there is no significant difference between the main quality components of C01-6Y01-47 and 02-42 and that of control potato. 2 the effect of low temperature stress on photosynthetic activity of transgenic potato plants with SPDS gene was determined. Chlorophyll content of potato plants transformed with SPDS gene during temperature stress, Net photosynthetic rate, stomatal conductance, intercellular CO2 concentration and transpiration rate showed that the chlorophyll contents of 01-47C01-49C02-57 and 02-72 were higher than those of the control potato plants. The net photosynthetic rate of the transgenic potato plants with SPDS gene was higher than that of the control plants, the stomatal conductance of the transgenic potato plants with SPDS gene was higher than that of the control potato plants, and the intercellular CO2 concentration of the transgenic potato plants with SPDS gene was lower than that of the control potato plants. The transpiration rate of transgenic potato with SPDS gene was higher than that of potato without control. It can be concluded that the photosynthetic activity of transgenic potato plants with SPDS gene is stronger than that of control potato plants. 3. The detection of cold tolerance of potato plants transferred with SPDS gene can be seen by measuring the electrical conductivity of leaves of potato plants transferred with SPDS gene at different low temperatures. The relative electrical conductivity of transgenic potato with SPDS gene increased more than 50 at -4 鈩，

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