本文選題：潰瘍性結(jié)腸炎 + 腸道真菌��； 參考：《中醫(yī)雜志》2017年24期

【摘要】：目的探討安腸湯治療潰瘍性結(jié)腸炎的可能作用機制。方法將112只大鼠隨機分為空白組、模型組、美沙拉嗪組、麗珠腸樂組和安腸湯高、中、低劑量組,每組16只。除空白組外其余各組大鼠建立潰瘍性結(jié)腸炎模型,造模后第10天空白組和模型組大鼠每只以生理鹽水3 ml灌胃;麗珠腸樂組以麗珠腸樂溶液(0.5億活菌/ml)灌胃,每次0.12 ml;美沙拉嗪組以美沙拉嗪混懸液(5 mg/ml)灌胃,每次0.35 ml;安腸湯低、中、高劑量組分別給予安腸湯濃縮液1、5、10 ml/(kg·d),每日1次,共10天。取結(jié)腸病變腸段進行HE染色觀察結(jié)腸黏膜組織形態(tài)學(xué),取各組糞便標(biāo)本應(yīng)用實時熒光定量技術(shù)測定大鼠腸道5種真菌含量(包括白色念珠菌、曲霉菌、青霉菌、枝孢菌、隱球菌)。結(jié)果 HE染色結(jié)果顯示,模型組大鼠結(jié)腸黏膜層幾乎完全缺損,潰瘍形成,黏膜充血糜爛,伴炎細(xì)胞浸潤;各治療組則較模型組出現(xiàn)改善。與空白組比較,其余各組大鼠腸道白色念珠菌、曲霉菌和青霉菌相對表達(dá)含量上明顯升高,而枝孢菌和隱球菌相對表達(dá)含量降低(P0.05)。與模型組比較,各治療組大鼠腸道白色念珠菌、曲霉菌和青霉菌相對表達(dá)含量明顯降低,枝孢菌和隱球菌相對表達(dá)含量升高(P0.05)。安腸湯中劑量組在降低曲霉菌及升高枝孢菌、隱球菌相對表達(dá)含量方面優(yōu)于安腸湯低、高劑量組,在降低白色念珠菌及青霉菌相對表達(dá)含量方面安腸湯高劑量組優(yōu)于安腸湯低、中劑量組(P0.05)。結(jié)論安腸湯對潰瘍性結(jié)腸炎大鼠腸道真菌具有明確的調(diào)節(jié)作用,可能是其治療潰瘍性結(jié)腸炎的作用機制之一。
[Abstract]:Objective to explore the possible mechanism of Anchang decoction in the treatment of ulcerative colitis. Methods 112 rats were randomly divided into blank group, model group, mesalazine group, Lizhu Changle group and Anchang decoction group with 16 rats in each group. The ulcerative colitis model was established in rats in each group except the blank group. After the establishment of the model, the rats in the 10th sky white group and the model group were given 3 ml normal saline, and the Lizhu Changle group was given Lizhu Changle solution (50 million live bacteria / ml), and the rats in the model group were given intragastric administration of Lizhu Changle solution (50 million live bacteria / ml). Each time 0.12 ml; the mesalazine group was perfused with 5 mg / ml of mesalazine suspension (0.35 ml / time); the low, medium and high dose group of Anchang decoction was given the concentrated solution of Anchang decoction for 10 days once a day. Colonic mucosal histomorphology was observed by HE staining, and the contents of 5 fungi (including Candida albicans, Aspergillus albicans, Penicillium and Cladosporium) were measured by real-time fluorescence quantitative technique. Cryptococcus sp. Results the results of HE staining showed that the colonic mucosa layer of the model group was almost completely defective, the ulcer formed, the mucosa hyperemia and erosion accompanied by inflammatory cell infiltration, and the treatment group was improved compared with the model group. Compared with the control group, the relative expression levels of Candida albicans, Aspergillus and Penicillium in the other groups were significantly increased, while the relative expression levels of Cladosporium and Cryptococcus were lower than those of the control group (P 0.05). Compared with the model group, the relative expression levels of Candida albicans, Aspergillus and Penicillium in the intestinal tract of rats in each treatment group were significantly decreased, while the relative expression levels of Cladosporium and Cryptococcus were increased (P 0.05). The relative expression of Cryptococcus was superior to that of Anchang decoction in decreasing Aspergillus and increasing Cladosporium, while in high dose group, the relative expression of Candida albicans and Penicillium was decreased in high dose group than in Anchang decoction group. Middle dose group (P 0.05). Conclusion Anchang decoction has a definite regulatory effect on intestinal fungi in rats with ulcerative colitis and may be one of the mechanisms of its therapeutic effect on ulcerative colitis.
【作者單位】： 廣西中醫(yī)藥大學(xué)第一附屬醫(yī)院仙葫院區(qū);柳州市中醫(yī)院;廣西中醫(yī)藥大學(xué);
【基金】：國家自然科學(xué)基金項目(81260542,81660795) 廣西中醫(yī)藥大學(xué)研究生教育創(chuàng)新計劃(YJS201635)
【分類號】：R285.5

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