本文選題：FABP + 心肌細(xì)胞　； 參考：《中國老年學(xué)雜志》2017年19期

【摘要】：目的脂肪酸結(jié)合蛋白(FABP)3基因?qū)π募〖?xì)胞凋亡的影響及機(jī)制。方法將H9C2心肌細(xì)胞分為空白對照組、陰性對照組、FABP3沉默組、FABP3沉默+空質(zhì)粒組及FABP3沉默+過表達(dá)人FABP3組,轉(zhuǎn)染72 h后Western印跡檢測各組細(xì)胞中FABP3的蛋白表達(dá);流式細(xì)胞儀檢測細(xì)胞凋亡;Western印跡檢測Cleaved caspase3、Bcl-2蛋白表達(dá)。結(jié)果空白對照組與陰性對照組及FABP3沉默組和FABP3沉默+空質(zhì)粒組之間FABP3蛋白表達(dá)差異無統(tǒng)計(jì)學(xué)意義(P0.05),FABP3沉默組和FABP3沉默+空質(zhì)粒組FABP3的蛋白表達(dá)顯著低于空白對照組與陰性對照組,FABP3沉默+過表達(dá)人FABP3組FABP3的蛋白表達(dá)顯著高于FABP3沉默組和FABP3沉默+空質(zhì)粒組(P0.01);FABP3沉默組和FABP3沉默+空質(zhì)粒組細(xì)胞凋亡率及Cleaved caspase3蛋白表達(dá)顯著高于空白對照組與陰性對照組,Bcl-2蛋白表達(dá)顯著低于空白對照組與陰性對照組FABP3(P0.01);沉默+過表達(dá)人FABP3組細(xì)胞凋亡率及Cleaved caspase3蛋白表達(dá)顯著低于FABP3沉默組和FABP3沉默+空質(zhì)粒組,Bcl-2蛋白表達(dá)顯著高于FABP3沉默組和FABP3沉默+空質(zhì)粒組(P0.01)。結(jié)論沉默F(xiàn)ABP3的表達(dá)可促進(jìn)H9C2心肌細(xì)胞凋亡,過表達(dá)則抑制細(xì)胞凋亡,其機(jī)制與調(diào)節(jié)Cleaved caspase3及Bcl-2蛋白表達(dá)有關(guān)。
[Abstract]:Objective to investigate the effect and mechanism of fatty acid binding protein (FABP3) gene on cardiomyocyte apoptosis. Methods H9C2 cardiomyocytes were divided into blank control group, negative control group and FABP3 silencing FABP3 group. The expression of FABP3 protein was detected by Western blot 72 h after transfection. Apoptosis was detected by flow cytometry. Western blot was used to detect the expression of Cleaved caspase3 and Bcl-2 protein. Results there was no significant difference in the expression of FABP3 protein between the blank control group and the negative control group, the FABP3 silencing group and the FABP3 silencing empty plasmid group. The expression of FABP3 protein in the blank control group and FABP3 silencing blank plasmid group was significantly lower than that in the blank control group. The expression of FABP3 protein in FABP3 group was significantly higher than that in FABP3 silencing group and FABP3 silencing empty plasmid group (P 0.01) and FABP3 silencing empty plasmid group. The apoptosis rate and Cleaved caspase3 protein expression in FABP3 silencing group and FABP3 silencing blank plasmid group were significantly higher than those in FABP3 silencing group and FABP3 silencing empty plasmid group. The expression of Bcl-2 protein in the control group and the negative control group was significantly lower than that in the blank control group and the negative control group, while the apoptosis rate and the expression of Cleaved caspase3 protein in the silent FABP3 group were significantly lower than those in the FABP3 silencing group and the FABP3 silencing empty plasmid group. White expression was significantly higher than that in FABP3 silencing group and FABP3 silencing empty plasmid group. Conclusion silencing the expression of FABP3 can promote the apoptosis of H9C2 cardiomyocytes, and over-expression can inhibit the apoptosis. The mechanism is related to the regulation of the expression of Cleaved caspase3 and Bcl-2 protein.
【作者單位】： 華中科技大學(xué)同濟(jì)醫(yī)學(xué)院附屬武漢中心醫(yī)院網(wǎng)絡(luò)醫(yī)療部;
【基金】：武漢市臨床醫(yī)學(xué)科研項(xiàng)目(WZ15D17)
【分類號】：R54

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