發(fā)布時(shí)間：2018-04-30 07:32

  本文選題：GCF + 肝細(xì)胞癌�。� 參考：《廣東醫(yī)學(xué)》2017年10期

【摘要】：目的探討沉默GCF2基因表達(dá)對人肝癌BEL-7404細(xì)胞侵襲能力的影響。方法實(shí)驗(yàn)分為GCF2-siRNA組、NC-siRNA組及MOCK組,GCF2-siRNA組轉(zhuǎn)染靶向GCF2基因的RNA干擾序列GCF2-siRNA,陰性對照組轉(zhuǎn)染陰性對照序列NC-siRNA,MOCK組不轉(zhuǎn)染任何序列。將靶向GCF2基因的RNA干擾序列(GCF2-siRNA)瞬時(shí)轉(zhuǎn)染BEL-7404細(xì)胞以沉默GCF2表達(dá),采用CCK-8法檢測細(xì)胞增殖活性,體外侵襲實(shí)驗(yàn)檢測細(xì)胞侵襲能力,Western blot檢測基質(zhì)金屬蛋白酶(MMP)-9及GCF2靶基因MMP-23蛋白表達(dá)。結(jié)果轉(zhuǎn)染GCF2-siRNA使GCF2表達(dá)下調(diào),后者導(dǎo)致BEL-7404增殖受阻;GCF2-siRNA組的穿膜細(xì)胞數(shù)較陰性對照組(NC-siRNA)及未轉(zhuǎn)染組(MOCK)明顯減少(穿膜細(xì)胞數(shù)分別為41.5±0.95、61.3±1.57、64.5±1.65,P0.05);MMP-9和MMP-23蛋白表達(dá)較兩個(gè)對照組明顯下降(P0.05)。結(jié)論下調(diào)GCF2表達(dá)能抑制BEL-7404侵襲能力,提示GCF2促進(jìn)肝癌細(xì)胞的侵襲可能是其參與肝癌發(fā)展進(jìn)程的重要途徑。
[Abstract]:Objective to investigate the effect of silent GCF2 gene expression on the invasiveness of human hepatocellular carcinoma BEL-7404 cells. Method experiments were divided into GCF2-siRNA group, NC-siRNA group and MOCK group, GCF2-siRNA group transfected RNA interference sequence of target GCF2 gene, negative control group transfected negative control sequence NC-siRNA, MOCK group did not transfect any sequence. The target GCF2 gene would be targeted. The RNA interference sequence (GCF2-siRNA) transiently transfected BEL-7404 cells to silence GCF2 expression, detected cell proliferation activity by CCK-8, and detected cell invasiveness in vitro, and Western blot detected the expression of MMP-23 protein of matrix metalloproteinase (MMP) -9 and GCF2 target gene. The number of membrane cells in the GCF2-siRNA group was significantly lower than that in the negative control group (NC-siRNA) and the untransfected group (MOCK) (the number of membrane cells were 41.5 + 0.95,61.3 + 1.57,64.5 + 1.65, P0.05), and the expression of MMP-9 and MMP-23 protein was significantly lower than that of the two control groups (P0.05). Conclusion the down-regulation GCF2 expression could inhibit BEL-7404 invasion ability, suggesting GCF2 promoted. Invasion of HCC cells may be an important way to participate in the development of HCC.

【作者單位】： 桂林醫(yī)學(xué)院組胚教研室;桂林醫(yī)學(xué)院臨床醫(yī)學(xué)院;
【基金】：廣西高�？蒲许�(xiàng)目(編號(hào):KY2015LX284) 大學(xué)生創(chuàng)新創(chuàng)業(yè)訓(xùn)練計(jì)劃項(xiàng)目(編號(hào):201410601011,201510601003)
【分類號(hào)】：R735.7
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本文編號(hào)：1823648