本文選題：轉(zhuǎn)復(fù)合多基因 + 歐美楊楊�。� 參考：《林業(yè)科學(xué)》2017年07期

【摘要】：【目的】通過觀測轉(zhuǎn)復(fù)合多基因歐美楊107楊(轉(zhuǎn)基因107楊)幼苗在不同濃度鹽脅迫下生長性狀,質(zhì)膜透性、光合作用等各項指標(biāo),以及外源基因表達產(chǎn)物積累量的變化,綜合評價轉(zhuǎn)復(fù)合多基因楊樹在鹽脅迫下的適應(yīng)能力。【方法】以轉(zhuǎn)化成功且已經(jīng)過驗證的轉(zhuǎn)基因107楊為試驗材料,未轉(zhuǎn)基因107楊為對照(CK),進行組培擴繁,在幼苗株高10 cm時定植在花盆中,置于人工氣候室中培養(yǎng)。分別用0、3、6 g·L~(-1)的Na Cl溶液進行鹽脅迫處理,脅迫25天后觀測2個株系幼苗的生長指標(biāo)、生理參數(shù)變化,以及甜菜堿與Bt毒蛋白的積累量。【結(jié)果】2個株系的生長指標(biāo)觀測結(jié)果表明:在低鹽濃度處理下,轉(zhuǎn)基因107楊幼苗株高增長量顯著高于非轉(zhuǎn)基因?qū)φ?轉(zhuǎn)基因株系幼苗地徑增長量與對照差異不顯著;高鹽濃度處理下,2個株系幼苗株高與地徑均受到鹽脅迫的顯著影響。2個株系的生理參數(shù)觀測結(jié)果表明:在低鹽濃度處理下,轉(zhuǎn)基因107楊幼苗質(zhì)膜透性顯著低于對照107楊,葉綠素含量有一定程度的增加,凈光合速率與蒸騰速率顯著高于對照,光系統(tǒng)Ⅱ?qū)嶋H光合效率Y(Ⅱ)小幅度降低但顯著高于對照,光系統(tǒng)Ⅱ能夠保持較高的電子傳遞速率;F_v/F_m增幅較高,具有較大光合作用潛力。在高鹽濃度下,轉(zhuǎn)基因株系幼苗葉綠素含量降幅較小,而質(zhì)膜透性、凈光合速率均顯著降低,F_v/F_m、Y(Ⅱ)均呈現(xiàn)較大幅度下降,光系統(tǒng)Ⅱ電子傳遞速率受到較大影響,光合能力下降,變化趨勢與對照相同,2個株系幼苗均受到較大程度的鹽害。轉(zhuǎn)基因株系中外源基因表達產(chǎn)物積累量的ELISA研究結(jié)果表明,隨鹽濃度的增加,Cry1Ac毒蛋白、Cry3A毒蛋白及甜菜堿含量都呈現(xiàn)增加趨勢;在高鹽濃度脅迫下外源基因產(chǎn)物積累量均顯著增加�！窘Y(jié)論】轉(zhuǎn)基因107楊在低濃度鹽脅迫下表現(xiàn)出良好的適應(yīng)性,耐鹽性優(yōu)于對照107楊。在高濃度鹽脅迫下轉(zhuǎn)基因株系與對照107楊均受到較大影響,轉(zhuǎn)基因株系并未顯示其優(yōu)勢。鹽脅迫可以誘導(dǎo)外源Bt基因、BADH基因表達加強,鹽脅迫下轉(zhuǎn)基因107楊表現(xiàn)出良好的耐鹽和抗蟲潛力。
[Abstract]:[objective] to observe the changes of growth traits, plasma membrane permeability, photosynthesis and accumulation of exogenous gene expression products in transgenic poplar 107 (transgenic poplar) seedlings under different concentrations of salt stress. The adaptability of transgenic poplar to salt stress was comprehensively evaluated. [methods] transgenic poplar 107, which had been successfully transformed and verified, was used as experimental material, and non-transgenic poplar 107 was used as control for tissue culture and propagation. The seedlings were planted in a flowerpot at a height of 10 cm and cultured in an artificial climate chamber. Salt stress was carried out in the NaCl solution of 0 ~ 3g / L ~ (-1). The growth indexes and physiological parameters of the seedlings of two strains were observed after 25 days of stress. And the accumulation of betaine and BT toxin protein. [results] the growth indexes of two lines were observed. The results showed that the growth of seedling height of transgenic poplar 107 was significantly higher than that of non-transgenic control under low salt concentration. Under high salt concentration, the height and diameter of two lines seedlings were significantly affected by salt stress. The physiological parameters of the two lines were observed under low salt concentration. The plasma membrane permeability of transgenic 107 poplar seedlings was significantly lower than that of the control, chlorophyll content increased to some extent, net photosynthetic rate and transpiration rate were significantly higher than that of the control, and the actual photosynthetic efficiency of light system 鈪，

本文編號：1808959