本文選題：卵巢腫瘤 + 骨髓　； 參考：《中國(guó)組織工程研究》2017年09期

【摘要】：背景:骨髓間充質(zhì)干細(xì)胞具有來(lái)源廣泛、免疫原性低等優(yōu)點(diǎn),尤其易于導(dǎo)入和表達(dá)外源基因,作為抗腫瘤基因治療的載體具有明顯優(yōu)越性。目的:探討骨髓間充質(zhì)干細(xì)胞負(fù)載白細(xì)胞介素12重組腺病毒對(duì)卵巢癌細(xì)胞增殖、細(xì)胞周期和細(xì)胞凋亡的影響。方法:腺病毒介導(dǎo)白細(xì)胞介素12基因轉(zhuǎn)染骨髓間充質(zhì)干細(xì)胞,RT-PCR和Western Blotting測(cè)定骨髓間充質(zhì)干細(xì)胞中白細(xì)胞介素12 m RNA和蛋白的表達(dá),ELISA法測(cè)定細(xì)胞上清液中白細(xì)胞介素12水平。將SKOV3卵巢癌細(xì)胞與白細(xì)胞介素12重組腺病毒轉(zhuǎn)染的骨髓間充質(zhì)干細(xì)胞上清液共培養(yǎng),對(duì)照組為SKOV3細(xì)胞與未轉(zhuǎn)染骨髓間充質(zhì)干細(xì)胞上清液共培養(yǎng),MTT法測(cè)定SKOV3細(xì)胞增殖活性,流式細(xì)胞儀檢測(cè)SKOV3細(xì)胞周期和細(xì)胞凋亡率。結(jié)果與結(jié)論:(1)腺病毒介導(dǎo)白細(xì)胞介素12基因成功轉(zhuǎn)染到骨髓間充質(zhì)干細(xì)胞中,轉(zhuǎn)染后細(xì)胞有白細(xì)胞介素12 m RNA和蛋白的表達(dá),空病毒載體組和空白對(duì)照組骨髓間充質(zhì)干細(xì)胞未檢測(cè)到白細(xì)胞介素12表達(dá);(2)培養(yǎng)48 h,白細(xì)胞介素12組骨髓間充質(zhì)干細(xì)胞上清液中白細(xì)胞介素12水平為(68.78±12.35)μg/L,空病毒載體組和空白對(duì)照組骨髓間充質(zhì)干細(xì)胞上清液中未檢測(cè)到白細(xì)胞介素12;(3)白細(xì)胞介素12轉(zhuǎn)染組骨髓間充質(zhì)干細(xì)胞上清液對(duì)SKOV3細(xì)胞增殖有抑制作用,細(xì)胞增殖抑制率隨時(shí)間的延長(zhǎng)而增加(P0.05)。轉(zhuǎn)染組SKOV3細(xì)胞G1期細(xì)胞比例高于對(duì)照組(P0.05),G2期細(xì)胞比例低于對(duì)照組(P0.05)。轉(zhuǎn)染組SKOV3細(xì)胞凋亡率高于對(duì)照組(P0.05);(4)結(jié)果表明,轉(zhuǎn)染白細(xì)胞介素12基因的骨髓間充質(zhì)干細(xì)胞能夠表達(dá)白細(xì)胞介素12,其培養(yǎng)上清液能夠抑制卵巢癌細(xì)胞增殖并誘導(dǎo)其凋亡。
[Abstract]:Background: bone marrow mesenchymal stem cells (BMSCs) have many advantages, such as wide source, low immunogenicity and so on. Aim: to investigate the effects of bone marrow mesenchymal stem cells loaded with interleukin 12 recombinant adenovirus on the proliferation, cell cycle and apoptosis of ovarian cancer cells. Methods: the expression of interleukin 12 RNA and protein in bone marrow mesenchymal stem cells were detected by RT-PCR and Western Blotting. The levels of interleukin 12 in supernatant of bone marrow mesenchymal stem cells were determined by Elisa. SKOV3 ovarian cancer cells were co-cultured with the supernatant of bone marrow mesenchymal stem cells transfected with interleukin-12 recombinant adenovirus, while SKOV3 cells and untransfected bone marrow mesenchymal stem cells supernatants were co-cultured to determine the proliferative activity of SKOV3 cells. SKOV3 cell cycle and apoptosis rate were measured by flow cytometry. Results and conclusion adenovirus-mediated interleukin-12 gene was successfully transfected into bone marrow mesenchymal stem cells, and IL-12 RNA and protein were expressed in the transfected cells. The level of interleukin 12 in the supernatant of bone marrow mesenchymal stem cells was 68.78 鹵12.35 渭 g / L after 48 h culture. The supernatant of bone marrow mesenchymal stem cells in body group and blank control group had no effect on the proliferation of SKOV3 cells in the supernatant of bone marrow mesenchymal stem cells (BMSCs), which was not detected in the supernatant of bone marrow mesenchymal stem cells (BMSCs). The inhibitory rate of cell proliferation increased with time. The percentage of SKOV3 cells in G1 phase in transfected group was higher than that in control group, which was lower than that in control group. The results showed that bone marrow mesenchymal stem cells transfected with interleukin-12 gene could express interleukin-12, and its supernatant could inhibit the proliferation and induce apoptosis of ovarian cancer cells.
【作者單位】： 河北醫(yī)科大學(xué)第一醫(yī)院;
【基金】：河北省2014年度醫(yī)學(xué)科學(xué)研究重點(diǎn)課題計(jì)劃項(xiàng)目(ZD20140325)~~
【分類號(hào)】：R737.31

【相似文獻(xiàn)】

相關(guān)期刊論文 前10條

1 朱妙珍,申海鷹,薛超;增殖性腎小球腎炎中白細(xì)胞介素8的表達(dá)及作用[J];中華腎臟病雜志;2000年06期

2 楊京平,洪天配,裴斐;白細(xì)胞介素18在慢性淋巴細(xì)胞性甲狀腺炎中的表達(dá)[J];診斷病理學(xué)雜志;2001年02期

3 徐建華,陸宇飛,孔令環(huán);白細(xì)胞介素8在老年慢性阻塞性肺疾病中的作用[J];中國(guó)老年學(xué)雜志;2004年04期

4 常志遠(yuǎn),徐荻;白細(xì)胞介素-2的研究進(jìn)展[J];中國(guó)處方藥;2004年08期

5 劉廣楨,顧覺奮,陳建華;白細(xì)胞介素-18[J];藥物生物技術(shù);2005年01期

6 石玉枝;劉豹;霍建民;張薇;;慢性阻塞性肺疾病易感性與白細(xì)胞介素1基因的多態(tài)性[J];中國(guó)臨床康復(fù);2006年16期

7 劉艷;黃元汛;;運(yùn)動(dòng)和營(yíng)養(yǎng)補(bǔ)充對(duì)白細(xì)胞介素的影響[J];中國(guó)臨床康復(fù);2006年44期

8 李昌明;肖青;韓建魁;;白細(xì)胞介素-6的臨床應(yīng)用進(jìn)展[J];中國(guó)誤診學(xué)雜志;2007年01期

9 潘建玲;關(guān)秀茹;馬學(xué)華;;白細(xì)胞介素-18在特發(fā)性血小板減少性紫癜中作用的研究[J];哈爾濱醫(yī)科大學(xué)學(xué)報(bào);2007年01期

10 鄧學(xué)軍;覃數(shù);;白細(xì)胞介素-10、白細(xì)胞介素-6在急性冠狀動(dòng)脈綜合征中的臨床意義[J];四川醫(yī)學(xué);2008年10期

相關(guān)會(huì)議論文 前10條

1 劉玉春;林士軍;葉建華;劉美描;陳雄;張靜;;白細(xì)胞介素2、可溶性白細(xì)胞介素2受體在慢性阻塞性肺疾病表達(dá)的研究[A];中華醫(yī)學(xué)會(huì)呼吸病學(xué)年會(huì)——2013第十四次全國(guó)呼吸病學(xué)學(xué)術(shù)會(huì)議論文匯編[C];2013年

2 曹艷華;張蕊;余奕;張威;祝成亮;王青;宋煜;白蘭;劉映樂;鄔開朗;吳建國(guó);;由白細(xì)胞介素-29介導(dǎo)的白細(xì)胞介素-27抑制乙型肝炎病毒復(fù)制機(jī)制的研究[A];2013年湖北省暨武漢微生物學(xué)會(huì)會(huì)員代表大會(huì)暨學(xué)術(shù)年會(huì)論文摘要集[C];2013年

3 姜曉君;魏海明;孫lm;田志剛;;白細(xì)胞介素2保護(hù)刀豆蛋白A誘導(dǎo)的肝損傷[A];第六屆全國(guó)免疫學(xué)學(xué)術(shù)大會(huì)論文集[C];2008年

4 張曉光;惠延年;韓泉洪;馬吉獻(xiàn);;培養(yǎng)人視網(wǎng)膜色素上皮細(xì)胞受附加磁場(chǎng)作用后單核細(xì)胞趨化蛋白-1和白細(xì)胞介素-8的表達(dá)[A];中國(guó)眼底病論壇·全國(guó)眼底病專題學(xué)術(shù)研討會(huì)論文匯編[C];2008年

5 曹艷華;吳建國(guó);;由白細(xì)胞介素-29介導(dǎo)的白細(xì)胞介素-27抑制乙型肝炎病毒復(fù)制機(jī)制的研究[A];2012年鄂粵微生物學(xué)學(xué)術(shù)年會(huì)——湖北省暨武漢微生物學(xué)會(huì)成立六十年慶祝大會(huì)論文集[C];2012年

6 鄭夢(mèng)凝;張湘燕;葉賢偉;余紅;李翠;;四種白細(xì)胞介素與阻塞性睡眠呼吸暫停低通氣綜合征[A];中華醫(yī)學(xué)會(huì)呼吸病學(xué)年會(huì)——2013第十四次全國(guó)呼吸病學(xué)學(xué)術(shù)會(huì)議論文匯編[C];2013年

7 李素，

本文編號(hào)：1805585