本文選題：縮骨鯽(Carassius + auratus　； 參考：《淡水漁業(yè)》2017年02期

【摘要】：采用同源克隆、生物信息學(xué)方法及熒光定量PCR(Real-time PCR)對(duì)縮骨鯽(Carassius auratus sogu var.)肌肉生長(zhǎng)抑制素(myostatin,MSTN)基因進(jìn)行克隆,分析和組織表達(dá)狀況研究。結(jié)果顯示:縮骨鯽基因組MSTN基因全長(zhǎng)4 737 bp,含有兩個(gè)內(nèi)含子和三個(gè)外顯子;編碼區(qū)長(zhǎng)度為1 128 bp,編碼375個(gè)氨基酸,包括信號(hào)肽(1aa~23aa),TGF-β前肽保守區(qū)域(34aa~256aa),TGF-β功能區(qū)(281aa~375aa),保守的RXXR蛋白酶酶切位點(diǎn)以及C-端活性區(qū)域9個(gè)保守的半胱氨酸殘基,這與其他物種MSTN相似。此外,縮骨鯽與鯽魚(Carassius auratus)的MSTN氨基酸序列同源性最高,達(dá)到96.1%。以β-actin為內(nèi)參基因,熒光定量PCR分析表明,MSTN在肌肉中表達(dá)量最高;腦、眼、心臟中次之;在肝胰臟、卵巢、腸和腎臟中微量表達(dá)。
[Abstract]:Homologous cloning, bioinformatics and fluorescence quantitative PCR (Real-time PCR) were used to clone, analyze and organize the gene of Carassius auratus sogu var. muscle somatostatin (myostatin, MSTN). The results showed that the total length of the MSTN gene of the genome of the Carassius auratus genome was 4737 BP, containing two introns and three exons. The length of the coding region is 1128 BP, encoding 375 amino acids, including signal peptide (1aa~23aa), TGF- beta propeptide conserved region (34aa~256aa), TGF- beta function region (281aa~375aa), conservative RXXR protease cutting site and C- terminal active region 9 conserved cysteine residues, which are similar to other species MSTN. In addition, the crucian carp and crucian carp (Carassius AUR) Atus) has the highest homology of MSTN amino acid sequence, reaching 96.1%. with beta -actin as the internal reference gene. Fluorescence quantitative PCR analysis shows that MSTN has the highest expression in the muscles; the brain, the eye, the heart, and the pancreas, the ovary, the intestines and the kidneys are expressed in the highest number.

【作者單位】： 廣州大學(xué)生命科學(xué)學(xué)院;韶關(guān)市水產(chǎn)研究所;華南農(nóng)業(yè)大學(xué)海洋學(xué)院;
【基金】：國(guó)家公益性行業(yè)(農(nóng)業(yè))重大專項(xiàng)(201303048) 廣東省科技計(jì)劃項(xiàng)目(2012A020602063,2014A020208145)
【分類號(hào)】：S917.4

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本文編號(hào)：1799071