發(fā)布時間：2018-04-24 13:33

  本文選題：癌 + 口腔　； 參考：《重慶醫(yī)科大學(xué)》2017年碩士論文

【摘要】：目的探討人口腔鱗癌SCC15細(xì)胞中生物鐘基因PER2的表達改變對細(xì)胞增殖、凋亡以及其它生物鐘基因的影響。方法通過構(gòu)建shRNA干擾人口腔鱗癌SCC15細(xì)胞中PER2基因;應(yīng)用流式細(xì)胞儀檢測口腔鱗癌細(xì)胞的增殖和凋亡水平的改變情況;實時熒光定量PCR檢測生物鐘基因CLOCK、BMAL1、PER1、PER3、DEC1、DEC2、CRY1、CRY2、TIM、CKIε、RORα、NPAS2和REV-ERBαmRNA表達。結(jié)果PER2沉默之后,SCC15癌細(xì)胞的凋亡降低,增殖加強(P0.05);SCC15細(xì)胞中生物鐘基因PER3、BMAL1、DEC1、DEC2、CRY2、TIM、RORα和NPAS2 mRNA的表達水平顯著降低,PER1和REV-ERBαmRNA的表達顯著升高(P0.05)。結(jié)論在癌細(xì)胞中,生物鐘基因PER2對生物鐘基因網(wǎng)絡(luò)中其它生物鐘基因具有重要調(diào)控作用,PER2表達降低導(dǎo)致細(xì)胞增殖增加和凋亡水平下降。
[Abstract]:Objective to investigate the effect of PER2 expression on cell proliferation, apoptosis and other clock genes in human oral squamous cell carcinoma (SCC15) cells. Methods shRNA was constructed to interfere with the expression of PER2 gene in human oral squamous cell carcinoma (SCC15) cells, flow cytometry was used to detect the changes of cell proliferation and apoptosis in oral squamous carcinoma cells, and real-time quantitative PCR was used to detect the expression of REV-ERB 偽 -NPAS2 and REV-ERB 偽 mRNA in human oral squamous cell carcinoma cells. Results after PER2 silencing, the apoptosis of SCC15 cells was decreased, and the expression of clock gene PER3, BMAL1, DEC2, CRY2, TIMROR 偽 and NPAS2 mRNA in SCC15 cells was significantly decreased. The expression of REV-ERB 偽 and PER1 was significantly increased in SCC15 cells. Conclusion in cancer cells, PER2 plays an important role in regulating the expression of other clock genes in the biological clock gene network. The decrease of PER2 expression leads to the increase of cell proliferation and the decrease of apoptosis.
【學(xué)位授予單位】：重慶醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：R739.8

【參考文獻】

相關(guān)期刊論文 前3條

1 葉華;楊凱;譚雪梅;趙丹;呂曉強;王青青;;鐘基因Per2和腫瘤相關(guān)鐘控基因在金黃地鼠口腔頰黏膜癌變不同階段的晝夜節(jié)律改變[J];華西口腔醫(yī)學(xué)雜志;2015年05期

2 林慶玲;蔡彥寧;袁艷鵬;左曉虹;;小鼠外周組織時鐘基因啟動子區(qū)甲基化分析[J];基礎(chǔ)醫(yī)學(xué)與臨床;2012年10期

3 丁健明;光照對哺乳類動物生物鐘的調(diào)節(jié)機制[J];基礎(chǔ)醫(yī)學(xué)與臨床;2005年07期

，

本文編號：1796851