本文選題：蘋(píng)果 + 磷酸蔗糖合酶��； 參考：《西北植物學(xué)報(bào)》2017年05期

【摘要】：磷酸蔗糖合酶(sucrose phosphate synthase,SPS)是植物中蔗糖合成的主要限速酶,影響植物的生長(zhǎng)發(fā)育和果實(shí)中蔗糖的含量。為探明蘋(píng)果中SPS基因家族特性及其在蔗糖合成中的作用,該研究從蘋(píng)果基因組中分離了MdSPS家族基因,分析了它們的進(jìn)化關(guān)系以及mRNA表達(dá)特性與酶活性和蔗糖含量的關(guān)系。結(jié)果顯示:(1)在蘋(píng)果基因組中有8個(gè)SPS家族基因表達(dá),它們分別屬于雙子葉植物的3個(gè)SPS亞家族。(2)熒光定量PCR分析顯示,蘋(píng)果C類(lèi)的MdSPS6基因和A類(lèi)的MdSPS1a/b基因是蘋(píng)果中表達(dá)豐度最高的SPS基因成員,其中MdSPS6在蘋(píng)果成熟果中表達(dá)豐度最高,其次是成熟葉片,而MdSPS1a/b在不積累蔗糖的幼果中表達(dá)豐度最高。(3)在果實(shí)發(fā)育過(guò)程中,除MdSPS1a/b之外,其它5個(gè)蘋(píng)果MdSPS家族基因均隨果實(shí)的生長(zhǎng)表達(dá)豐度增加,與SPS活性和蔗糖含量明顯呈正相關(guān)關(guān)系。研究表明,C類(lèi)家族MdSPS6是蘋(píng)果果實(shí)發(fā)育后期和葉片中蔗糖合成的主要SPS基因。
[Abstract]:Sucrose phosphate synthase (SPSs) is the main rate-limiting enzyme for sucrose synthesis in plants, which affects the growth and development of plants and the content of sucrose in fruits. In order to investigate the characteristics of SPS gene family in apple and its role in sucrose synthesis, MdSPS family genes were isolated from apple genome, and their evolutionary relationship and the relationship between mRNA expression characteristics and enzyme activity and sucrose content were analyzed. The results showed that there were 8 SPS family genes expressed in apple genome, which belonged to three SPS subfamilies of dicotyledonous plants. MdSPS6 gene of class C and MdSPS1a/b gene of class A are the most abundant members of SPS gene in apple, and MdSPS6 is the most abundant in mature apple fruit, followed by mature leaf. However, the expression of MdSPS1a/b was the highest in young fruits without sucrose accumulation. During fruit development, all the other five MdSPS family genes except MdSPS1a/b increased with the growth of fruit, which was positively correlated with the activity of SPS and the content of sucrose. The results showed that MdSPS6 of C family was the main SPS gene of sucrose synthesis in apple fruit.
【作者單位】： 西北農(nóng)林科技大學(xué)園藝學(xué)院;
【基金】：國(guó)家自然科學(xué)基金(31372038)
【分類(lèi)號(hào)】：S661.1
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本文編號(hào)：1791658