本文選題：鉛 + 鎘　； 參考：《中南林業(yè)科技大學(xué)學(xué)報(bào)》2017年08期

【摘要】：為篩選適用于Pb與Cd脅迫下旱柳實(shí)時(shí)熒光定量PCR的內(nèi)參基因,應(yīng)用熒光定量PCR技術(shù)分析UBQ1、TUB6、Actin、EF1α、18SRNA、GAPDH、TUA8和UBQ2這8個(gè)內(nèi)參基因在旱柳根部的表達(dá)情況。通過Normfinder、ge Norm和Best Keeper3個(gè)軟件進(jìn)行綜合分析,發(fā)現(xiàn)在Pb與Cd脅迫下表達(dá)較為穩(wěn)定的內(nèi)參基因均為EF1α、GAPDH和Actin,差別是在Pb脅迫下的旱柳根組織中最為穩(wěn)定的內(nèi)參基因是EF1α,而在Cd脅迫下的旱柳根組織應(yīng)最穩(wěn)定的內(nèi)參基因?yàn)镚APDH。該研究結(jié)果可用于旱柳根組織在Pb、Cd脅迫下基因表達(dá)的進(jìn)一步研究。
[Abstract]:In order to screen the internal reference genes suitable for real-time quantitative PCR of Salix willow under Pb and CD stress, the expression of eight internal reference genes (UBQ1, TUB6, EF1 偽 ~ (18) SRNAD, TUA8 and UBQ2) in the root of Salix davidii was analyzed by fluorescence quantitative PCR. Through the analysis of Normfinderge Norm and Best Keeper3 software, It was found that the stable endogenous reference genes expressed under Pb and CD stress were EF1 偽 GAPDH and Actin. the difference was that the most stable endogenous reference gene was EF1 偽 in root tissue of Salix wilfordii under Pb stress, but the most stable endogenous reference gene was GAPDH in root tissue under CD stress. The results can be used to further study the gene expression in the root tissues of Salix wilfordii under Pb and CD stress.
【作者單位】： 中南林業(yè)科技大學(xué)生命科學(xué)與技術(shù)學(xué)院;中南林業(yè)科技大學(xué)環(huán)境科學(xué)與工程學(xué)院;
【基金】：湖南省重點(diǎn)研發(fā)計(jì)劃項(xiàng)目“微生物-植物協(xié)同修復(fù)土壤重金屬污染及配套支撐技術(shù)”(2016SK2030)
【分類號(hào)】：S792.12
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本文編號(hào)：1789977