本文選題：紅麻 + 耐鹽　； 參考：《農(nóng)業(yè)生物技術(shù)學(xué)報》2017年12期

【摘要】：WD-重復(fù)蛋白(WD-repeat protein)含有WD-40基序,又稱為WD40重復(fù)蛋白,在植物對非生物逆境脅迫響應(yīng)過程中具有重要的調(diào)控作用。紅麻(Hibiscus cannabinus)是重要的工業(yè)紡織原料,是鹽堿地土壤改良的先鋒作物。本研究以紅麻轉(zhuǎn)錄組中與WD40基因高度相似的Unigene為參考,設(shè)計引物,進行反轉(zhuǎn)錄PCR擴增,經(jīng)Sanger測序獲得基因Hc WD40-1(Gen Bank登錄號:KX711617)的c DNA序列,生物信息學(xué)分析表明,該基因開放閱讀框為1 356 bp,編碼451個氨基酸,具有7個典型的WD40結(jié)構(gòu)域。q RT-PCR分析表明,Hc WD40-1基因受鹽和干旱脅迫誘導(dǎo)表達,同時受脫落酸(abscisic acid,ABA)和茉莉酸甲酯(methyl jasmonate,Me JA)的誘導(dǎo)。研究結(jié)果表明,Hc WD40-1基因是ABA和Me JA信號轉(zhuǎn)導(dǎo)途徑、鹽和干旱脅迫應(yīng)答途徑的關(guān)鍵樞紐基因。該研究結(jié)果為紅麻耐鹽、抗旱的脫落酸信號調(diào)控網(wǎng)絡(luò)和茉莉酸信號調(diào)控網(wǎng)絡(luò)的研究提供基礎(chǔ)資料。
[Abstract]:WD- repeat protein contains WD-40 motif, also known as WD40 repeat protein, which plays an important role in plant response to abiotic stress. Hibiscus cannabinus is an important industrial textile material and a pioneer crop for soil improvement in saline-alkali soil. In this study, the highly similar Unigene gene of kenaf transcriptiongroup was used as reference, primers were designed and reverse transcription PCR was carried out. The c DNA sequence of the gene HC WD40-1(Gen Bank accession number: KX711617 was obtained by Sanger sequencing. Bioinformatics analysis showed that, The open reading frame of the gene was 1 356 BP, encoding 451 amino acids, with seven typical WD40 domains. Q RT-PCR analysis showed that the Hc WD40-1 gene was induced by salt and drought stress, and was also induced by Aba (abscisic acidida) and methyl jasmonate (me JAA). The results showed that the Hc WD40-1 gene was the key gene of ABA and me JA signal transduction pathway, and the key gene of salt and drought stress response pathway. The results provided the basic data for the study of salt tolerance, drought resistance abscisic acid signal control network and jasmonic acid signal control network.
【作者單位】： 湖南文理學(xué)院生命與環(huán)境科學(xué)學(xué)院;中國農(nóng)業(yè)科學(xué)院麻類研究所;
【基金】：國家麻類產(chǎn)業(yè)技術(shù)體系紅麻育種項目(No.CARS-19-E07) 中國農(nóng)業(yè)科學(xué)院科技創(chuàng)新工程一年生麻類育種項目(No.ASTIP-IBFC03)
【分類號】：Q943.2;S563.5
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本文編號：1784764