發(fā)布時間：2018-04-17 21:55

  本文選題：番茄 + TIFY家族��； 參考：《分子植物育種》2017年04期

【摘要】：TIFY蛋白是一類植物特有的轉(zhuǎn)錄因子,在植物的生長發(fā)育、信號傳導(dǎo)以及脅迫反應(yīng)中起重要作用。盡管一些模式植物TIFY家族基因已經(jīng)被鑒定,但在茄科植物中有關(guān)TIFY家族基因的研究卻很少。本研究利用生物信息學(xué)手段分別從番茄與馬鈴薯中挖掘到21個Sl TIFY和21個St TIFY基因,并系統(tǒng)性分析這些TIFY基因的序列、表達以及進化特性。染色體定位與擴增模式分析顯示,番茄和馬鈴薯的TIFY基因散落分布在不同染色體上,家族成員擴增主要由串聯(lián)和片段重復(fù)產(chǎn)生。序列分析表明:番茄TIFY基因結(jié)構(gòu)被分為12種,馬鈴薯TIFY基因結(jié)構(gòu)被分為8種;番茄和馬鈴薯TIFY蛋白共享了保守基序1,其它保守基序顯示不同程度基因特異性,保守基序組成模式表現(xiàn)出高度多樣化。系統(tǒng)進化分析顯示,番茄和馬鈴薯TIFY蛋白被分為7大類群,同類群內(nèi)結(jié)構(gòu)與序列相似性較高。選擇壓力檢測表明,番茄的10對同源基因和馬鈴薯的19對同源基因均受到負選擇作用。表達譜結(jié)果表明,番茄和馬鈴薯TIFY基因的表達呈現(xiàn)較高的多樣性,這可能與多樣化的功能相關(guān)。這些研究結(jié)果為功能解析番茄和馬鈴薯TIFY基因提供理論指導(dǎo)。
[Abstract]:TIFY protein is a kind of plant specific transcription factor, which plays an important role in plant growth and development, signal transduction and stress response.Although some model plant TIFY family genes have been identified, few studies on TIFY family genes have been carried out in eggplant plants.In this study, 21 SL TIFY and 21 St TIFY genes were extracted from tomato and potato by bioinformatics, and the sequence, expression and evolution of these TIFY genes were analyzed systematically.Chromosome localization and amplification pattern analysis showed that the TIFY genes of tomato and potato were scattered on different chromosomes and the family members were mainly produced by tandem and fragment repetition.Sequence analysis showed that tomato TIFY gene structure was divided into 12 species, potato TIFY gene structure was divided into 8 species, tomato and potato TIFY protein shared conserved motif 1, other conserved motifs showed different degree of gene specificity.The conserved motif composition model is highly diversified.Phylogenetic analysis showed that the TIFY protein of tomato and potato were divided into 7 groups, and the structure and sequence of the same group were similar.The selection pressure test showed that 10 pairs of homologous genes of tomato and 19 pairs of homologous genes of potato were negatively selected.The results of expression profile showed that the expression of TIFY gene in tomato and potato showed high diversity, which may be related to the diversity of function.These results provide theoretical guidance for functional analysis of TIFY gene in tomato and potato.
【作者單位】： 周口師范學(xué)院生命科學(xué)與農(nóng)學(xué)學(xué)院;周口師范學(xué)院河南省作物分子育種與生物反應(yīng)器重點實驗室;
【基金】：河南省教育廳項目(17B180009) 周口師范學(xué)院2014年高層次人才科研啟動經(jīng)費資助項目(ZKNU2014109) 周口師范學(xué)院2015年度校本項目(ZKNUB215103)共同資助
【分類號】：S532;S641.2
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本文編號：1765438