溫度對裂殖壺菌S31中FAS及PKS基因轉(zhuǎn)錄水平的影響
發(fā)布時間:2018-04-17 18:05
本文選題:裂殖壺菌 + DHA; 參考:《中國油脂》2017年08期
【摘要】:以甘油為碳源,測定了搖瓶中裂殖壺菌S31進(jìn)入脂質(zhì)積累期后,溫度對胞內(nèi)油脂脂肪酸組成以及FAS和PKS基因轉(zhuǎn)錄水平的影響。結(jié)果表明:當(dāng)裂殖壺菌進(jìn)入脂質(zhì)積累階段后,提高發(fā)酵溫度到30℃會減少藻油中DHA的含量,FAS基因的轉(zhuǎn)錄水平比PKS基因的更高;同時,降低發(fā)酵溫度到20℃會提高裂殖壺菌S31藻油DHA的含量,PKS基因的轉(zhuǎn)錄水平顯著高于FAS基因的。實驗結(jié)果表明,溫度對PKS和FAS基因的轉(zhuǎn)錄水平有顯著的影響,從而決定裂殖壺菌S31中藻油脂肪酸組成的變化。
[Abstract]:Using glycerol as carbon source, the effects of temperature on the fatty acid composition of intracellular lipid and the transcription level of FAS and PKS genes were measured after S31 entered the lipid accumulation period in shaking flask.The results showed that when merozoites entered the stage of lipid accumulation, increasing fermentation temperature to 30 鈩,
本文編號:1764680
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