本文選題：玉米 + BADH基因�。� 參考：《玉米科學》2016年03期

【摘要】：以轉(zhuǎn)來自耐鹽植物異苞濱藜的甜菜堿醛脫氫酶基因(BADH)玉米T8代株系BZ-136及受體對照自交系鄭58(耐鹽)為試材,采用盆栽種植方法,分析轉(zhuǎn)基因植株中外源基因的表達情況,檢測耐鹽相關(guān)生理指標。結(jié)果表明,轉(zhuǎn)基因株系BZ-136中的BADH酶活性及甜菜堿含量顯著高于受體對照,隨著鹽脅迫時間的延長,其表達量先增加后減少,在脅迫7 d時分別達到了最大值。轉(zhuǎn)基因株系幼苗株高和干重從鹽脅迫第3天開始顯著高于對照,鮮重和含水量在脅迫第5天和第7天時轉(zhuǎn)基因株系顯著高于對照;轉(zhuǎn)基因株系根總體積顯著高于對照,直徑和根尖數(shù)在脅迫第5天時顯著高于對照;轉(zhuǎn)基因株系電導率和丙二醛含量均低于對照,分別在脅迫第3天和第7天開始達顯著差異,葉綠素含量高于受體對照,從第3天開始二者差異達顯著水平。由于外源BADH基因的表達顯著提高了基因株系的耐鹽性。
[Abstract]:The transgenic maize T8 strain BZ-136 and the receptor inbred line Zheng 58 (salt tolerance) were used to analyze the expression of foreign genes in transgenic plants by pot planting.The physiological indexes related to salt tolerance were detected.The results showed that the activity of BADH enzyme and the content of betaine in BZ-136 were significantly higher than those in the receptor control. With the prolongation of salt stress time, the expression of BADH increased first and then decreased, and reached the maximum value at 7 days of stress.The plant height and dry weight of transgenic lines were significantly higher than that of the control from the third day of salt stress, the fresh weight and water content were significantly higher than that of the control on the 5th and 7th day of stress, the total root volume of the transgenic lines was significantly higher than that of the control, and the total root volume of the transgenic lines was significantly higher than that of the control.The diameter and the number of root tips were significantly higher than that of the control on the 5th day, the electrical conductivity and malondialdehyde content of the transgenic lines were lower than those of the control, and the chlorophyll content was higher than that of the receptor control at the 3rd and 7th day of stress, respectively.From the third day, the difference between the two reached a significant level.The expression of exogenous BADH gene significantly improved the salt tolerance of the lines.
【作者單位】： 東北農(nóng)業(yè)大學農(nóng)學院;
【基金】：轉(zhuǎn)基因重大專項“轉(zhuǎn)基因玉米小麥大豆環(huán)境安全評價技術(shù)”(2015ZX08011003) 東北農(nóng)業(yè)大學科學研究基金項目(2012RCB21)
【分類號】：S513
，

本文編號：1758069