100個小麥品種資源抗條銹性鑒定及重要抗條銹病基因的SSR檢測
發(fā)布時間:2018-04-16 06:57
本文選題:小麥條銹病 + 抗病性鑒定; 參考:《植物保護》2017年02期
【摘要】:了解小麥品種資源對中國條銹菌生理小種的抗病性水平及其所含重要抗條銹病基因,可為合理種植和利用小麥品種提供理論依據(jù)。選用中國小麥條銹菌不同生理小種CYR17、CYR32、CYR33和V26及條銹菌混合小種分別對100個小麥品種資源進行苗期和成株期抗條銹性鑒定,并采用SSR分子標(biāo)記技術(shù)檢測重要抗條銹病基因Yr5、Yr10、Yr15、Yr18、Yr24和Yr26。結(jié)果表明,在苗期對4個生理小種均表現(xiàn)抗病性的有‘Mos311’、‘蘭天15號’等30個品種;在成株期表現(xiàn)抗病性的有‘Yeoman’、‘蘭天1號’、‘小紅麥’等88個品種;苗期和成株期均抗病的有‘Mos311’、‘蘭天15號’等30個品種。SSR檢測發(fā)現(xiàn),‘貴農(nóng)22’可能含有Yr5,‘蘭天23號’、‘蘭天24’號、‘中梁04260’和‘中梁04335’可能含有Yr10,‘Little Joss’和‘中梁5號’可能含有Yr15,‘清農(nóng)3號’、‘蘭天2號’、‘中梁04335’等19個品種可能含有Yr18,檢測品種可能均不含Yr24和Yr26。在鑒定中保持穩(wěn)定抗病性的‘Mos311’、‘蘭天15號’等8個品種在抗病品種選育中有重要應(yīng)用價值。
[Abstract]:Understanding the resistance level of wheat variety resources to physiological races of Chinese stripe rust and its important genes of resistance to stripe rust can provide a theoretical basis for the rational planting and utilization of wheat varieties.100 wheat cultivars were selected to identify their resistance to stripe rust at seedling stage and adult stage, respectively, using different physiological races CYR17, CYR32, CYR33 and V26 and mixed races of stripe rust, respectively, and SSR molecular marker technique was used to detect the important genes Yr5, Yr10, Yr15Yr18Yr24 and Yr26.The results showed that there were 30 varieties with disease resistance to four physiological races at seedling stage, such as Mos311Che 'Lantian 15', and 88 varieties such as Yeomanzao 'Lantian' No.1 'Gongmanniao', etc., which showed disease resistance at adult plant stage.In seedling and adult stage, 30 varieties, I. e., Mos311Con 'Lantian 15' and so on, were tested by SSR. It was found that 'Guinong 22' may contain Yr5 'Nong' Lantian 23 'Guang' Lantian 24'.'Zhongliang 04260' and' Zhongliang 04335' may contain Yr10Pu Little Joss' and 'Zhongliang 5' may contain Yr15Nong 'Qingnong' No.3 'Qingnong No.3' Zhongnong'2 'Zhongliang 04335' and other 19 varieties may contain Yr18.The tested varieties may not contain Yr24 or Yr26.Eight varieties, such as Mos311Che 'Lantian 15', which maintain stable disease resistance in identification, have important application value in the breeding of disease-resistant varieties.
【作者單位】: 青海大學(xué)農(nóng)牧學(xué)院;中國農(nóng)業(yè)科學(xué)院植物保護所植物病蟲害生物學(xué)國家重點實驗室;青海省農(nóng)林科學(xué)院青海省農(nóng)業(yè)有害生物綜合治理重點實驗室;農(nóng)業(yè)部西寧作物有害生物科學(xué)觀測實驗站;
【基金】:國家自然科學(xué)基金(31261140370,31272033) 國家重點基礎(chǔ)研究發(fā)展計劃(“973”計劃)(2013CB127700) “十二五”國家科技支撐計劃(2012BAD19B04) 農(nóng)業(yè)部作物種質(zhì)資源保護子項目(2016NWB036-12)
【分類號】:S435.121.42
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