本文選題：大白菜 + 雄性不育基因　； 參考：《分子植物育種》2017年02期

【摘要】：雜種優(yōu)勢(shì)的利用是提高白菜類(lèi)作物產(chǎn)量的一個(gè)重要途徑,也是遺傳育種工作者集中研究的領(lǐng)域。為克服常規(guī)制種缺點(diǎn),本課題組長(zhǎng)期致力于雄性不育材料的創(chuàng)制,并積極在細(xì)胞和分子水平上分析其遺傳規(guī)律。本次試驗(yàn)以大白菜核基因控制的雄性不育甲型兩用系"AB01"(本課題組自主創(chuàng)制)可育植株和不育植株各級(jí)花蕾為材料,利用抑制消減雜交方法構(gòu)建正反cDNA文庫(kù),并從中篩選出一個(gè)大白菜育性相關(guān)基因1F203片段;并利用白菜數(shù)據(jù)庫(kù)及NCBI對(duì)該片段進(jìn)行同源比對(duì)分析,確定其為SKS基因家族中的一員;同時(shí)利用RT-PCR技術(shù)對(duì)該片段進(jìn)行了時(shí)空表達(dá)分析,明確其在可育植株成熟花粉中有較高表達(dá)。為接下來(lái)克隆該片段對(duì)應(yīng)基因的全長(zhǎng)序列,分析其生物學(xué)信息,研究其蛋白質(zhì)功能提供幫助。
[Abstract]:The utilization of heterosis is an important way to improve the yield of Chinese cabbage, and is also the field of genetic and breeding researchers.In order to overcome the shortcomings of conventional seed production, our team has been devoted to the creation of male sterile materials for a long time, and actively analyzed their genetic laws at the cellular and molecular levels.In this experiment, we constructed a positive and negative AB01 library by suppression subtractive hybridization from fertile plants and flower buds of sterile plants of "AB01", a dual-purpose male sterile line controlled by nuclear gene of Chinese cabbage.A 1F203 fragment of the fertility related gene of Chinese cabbage was screened, and a member of the SKS gene family was identified by using the Chinese cabbage database and NCBI to analyze the homology of the fragment.At the same time, the RT-PCR technique was used to analyze the temporal and spatial expression of the fragment, and it was confirmed that the fragment was highly expressed in the mature pollen of fertile plants.It is helpful to clone the full length sequence of the corresponding gene, analyze its biological information and study its protein function.
【作者單位】： 沈陽(yáng)農(nóng)業(yè)大學(xué)遼寧省十字花科蔬菜遺傳育種重點(diǎn)實(shí)驗(yàn)室;
【基金】：國(guó)家自然基金(31272157) 遼寧自然科學(xué)基金(2013020071)共同資助
【分類(lèi)號(hào)】：S634.1;Q943.2

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本文編號(hào)：1753597