本文選題：油桐 + 乙醛脫氫酶　； 參考：《植物生理學(xué)報(bào)》2017年08期

【摘要】：本研究運(yùn)用反轉(zhuǎn)錄PCR(reverse transcription PCR,RT-PCR)技術(shù),從油桐(Vernicia fordii)近成熟種子中克隆了油桐乙醛脫氫酶基因家族兩個(gè)成員的全長(zhǎng)編碼序列(CDS),命名為VfALDH2B4和VfALDH2B7,NCBI登記號(hào)分別為MF179125和MF179126,CDS長(zhǎng)度分別為1 617和1 626 bp,分別編碼538和541個(gè)氨基酸。通過(guò)蛋白結(jié)構(gòu)分析發(fā)現(xiàn)這兩個(gè)蛋白均含有乙醛脫氫酶基因家族特有的保守結(jié)構(gòu)域,且兩個(gè)蛋白都與麻瘋樹(shù)(Jatropha curcas)的ALDH2B4和ALDH2B7親緣關(guān)系最近。實(shí)時(shí)熒光定量PCR分析表明,這兩個(gè)基因在油桐各組織器官中均有表達(dá),其中VfALDH2B4在嫩葉的表達(dá)量最高,是其他組織的21倍左右,而f ALDH2B7在花瓣的表達(dá)量最高,是其他組織的62倍左右;在油桐種子不同發(fā)育時(shí)期,兩個(gè)基因的表達(dá)也存在明顯差異。此外,兩個(gè)基因表達(dá)還受高鹽、干旱和脫落酸調(diào)控,它們可能在油桐逆境脅迫應(yīng)答和脫落酸信號(hào)途徑中發(fā)揮作用。
[Abstract]:In this study, reverse transcription PCR(reverse transcription PCR RT PCR was used.The full-length coding sequence of VfALDH2B4 and VfALDH2B7NCBI was cloned from the nearly mature seeds of Vernicia fordii. The length of MF179125 and MF179126 CDS were 1 617 and 1 626 BP, respectively, encoding 538 and 541 amino acids, respectively.It was found that the two proteins contained the conserved domain of acetaldehyde dehydrogenase gene family, and the two proteins were closely related to the ALDH2B4 and ALDH2B7 of Jatropha curcasa.Real-time fluorescence quantitative PCR analysis showed that the two genes were expressed in all tissues and organs of Taulownia. The expression of VfALDH2B4 was the highest in young leaves, about 21 times of that in other tissues, and the expression of f ALDH2B7 in petals was the highest.The expression of the two genes was also significantly different in different development stages of the seeds of Taulownia oleifera, and the expression of the two genes was also significantly different from that of other tissues.In addition, the two genes are also regulated by high salt, drought and abscisic acid, which may play a role in the response to stress and the signaling pathway of abscisic acid.
【作者單位】： 中南林業(yè)科技大學(xué)經(jīng)濟(jì)林培育與保護(hù)省部共建教育部重點(diǎn)實(shí)驗(yàn)室;
【基金】：湖南省科技計(jì)劃項(xiàng)目(2016NK2147)~~
【分類號(hào)】：S794.3
，

本文編號(hào)：1746586