本文選題：中華水韭 + LEAFY基因　； 參考：《植物科學(xué)學(xué)報(bào)》2017年01期

【摘要】：利用核基因LEAFY第二個(gè)內(nèi)含子片段對中國現(xiàn)存中華水韭(Isoetes sinensis Palmer)的遺傳多樣性進(jìn)行分析,探討了中華水韭自然居群的遺傳多樣性結(jié)構(gòu)及其形成機(jī)制。結(jié)果顯示:現(xiàn)存中華水韭7個(gè)自然居群共105個(gè)樣本中存在78個(gè)單倍型,單倍型多樣性(H_d)為0.989,核苷酸多樣性(π)為0.021,遺傳差異主要存在于居群內(nèi)(72%),且存在較高的基因流(N_m=0.59)。同時(shí),居群遺傳學(xué)分析結(jié)果發(fā)現(xiàn)中華水韭居群不存在明顯的譜系地理格局(G_(ST)N_(ST));Mantel檢驗(yàn)中Rxy值為-0.286,P(rxy-rand≥rxy-data)值為0.370,表明居群遺傳距離和地理距離之間沒有明顯相關(guān)性;UPGMA聚類分析顯示處于海拔較高位置的2個(gè)居群與其它5個(gè)居群遺傳關(guān)系較遠(yuǎn);中性檢驗(yàn)(Taijima's D、FuLi's D*和F*)檢測結(jié)果均為負(fù)值,基于穩(wěn)定模型的失配分布檢測顯示為多峰。根據(jù)中華水韭居群的地理位置,推測中華水韭的遺傳結(jié)構(gòu)可能與水系、海拔分布及其雜交后代多倍化的物種形成過程相關(guān)。
[Abstract]:The genetic diversity of Isoetes sinensis Palmer in China was analyzed by using the second intron fragment of nuclear gene LEAFY, and the genetic diversity structure and formation mechanism of the natural population of Isoetes chinensis were discussed.The results showed that there were 78 haplotypes (Hd) and 0.021 nucleotide diversity (蟺) in 105 samples of 7 natural populations of leek sinensis.At the same time,The results of population genetics analysis showed that there was no obvious phylogenetic pattern in the population of Allium sinensis, and the Rxy value of Mantel test was -0.286 Prxy-rand 鈮，

本文編號(hào)：1745489