本文選題：肝癌　切入點(diǎn)：KIFA　出處：《現(xiàn)代腫瘤醫(yī)學(xué)》2016年24期

【摘要】：目的:研究KIF20A對肝癌SMMC-7721細(xì)胞系增殖和侵襲遷移能力的影響。方法:利用RNA干擾技術(shù)下調(diào)肝癌細(xì)胞系SMCC-7721中KIF20A的表達(dá)。qRT-PCR和Western blot分別檢測細(xì)胞KIF20A在mRNA和蛋白水平表達(dá)中的變化。CCK-8實(shí)驗(yàn)檢測增殖能力的改變。Transwell實(shí)驗(yàn)檢測遷移、侵襲能力的改變。結(jié)果:qRT-PCR和Western blot結(jié)果顯示KIF20A-siRNA能夠成功下調(diào)KIF20A的表達(dá),CCK-8和Transwell實(shí)驗(yàn)分別證實(shí)肝癌SMMC-7721細(xì)胞增殖和侵襲遷移能力下降。結(jié)論:抑制KIF20A的表達(dá)能夠降低肝癌細(xì)胞的增殖和侵襲遷移能力。
[Abstract]:Objective: to study the effect of KIF20A on the proliferation, invasion and migration of hepatocellular carcinoma SMMC-7721 cell line.Methods: the expression of KIF20A in hepatocellular carcinoma cell line SMCC-7721 was down-regulated by RNA interference technique. QRT-PCR and Western blot were used to detect the changes of KIF20A expression at mRNA and protein levels respectively. CCK-8 assay was used to detect the change of proliferation ability. Transwell assay was used to detect migration and invasion ability.Results the results of KIF20A-siRNA and Western blot showed that KIF20A-siRNA could successfully down-regulate the expression of KIF20A. CCK-8 and Transwell experiments confirmed that the proliferation, invasion and migration of SMMC-7721 cells decreased respectively.Conclusion: inhibiting the expression of KIF20A can reduce the proliferation, invasion and migration of hepatoma cells.
【作者單位】： 榆林市第一醫(yī)院消化內(nèi)科;榆林市第一醫(yī)院婦產(chǎn)科;第四軍醫(yī)大學(xué)航空航天醫(yī)學(xué)系;
【基金】：國家自然科學(xué)基金青年項(xiàng)目(編號:81301681)
【分類號】：R735.7
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本文編號：1721586