本文選題：龍眼　切入點(diǎn)：果肉自溶　出處：《熱帶作物學(xué)報(bào)》2017年05期

【摘要】：果肉自溶是影響龍眼采后果實(shí)貯運(yùn)、貨架壽命和貯藏品質(zhì)的重要因素。本研究通過同源序列比對及PCR技術(shù)在龍眼果肉中克隆獲得與龍眼自溶相關(guān)基因cDNA序列,命名為DlMC4。同源比對及系統(tǒng)進(jìn)化樹分析結(jié)果表明,DlMC4與荔枝LcMC氨基酸序列的相似性最高。定量結(jié)果表明:在采后常溫貯藏期間,果肉自溶指數(shù)隨DlMC4基因表達(dá)量的上升逐步升高,且DlMC4基因在自溶指數(shù)較高的‘東壁’果肉中表達(dá)量高于自溶指數(shù)較低的‘石硤’果肉。果肉中Caspase-4酶活性的分析結(jié)果表明,2個(gè)品種的DlMC4基因表達(dá)與其酶活性和果肉自溶指數(shù)呈顯著正相關(guān)。據(jù)此推測,采后貯藏后期果肉中DlMC4基因的上調(diào)表達(dá)可能對其果肉自溶起促進(jìn)作用。
[Abstract]:Flesh autolysis is an important factor affecting fruit storage and transportation, shelf life and storage quality of longan.In this study, the homologous sequence alignment and PCR technique were used to clone the cDNA sequence of longan autolytic gene from longan pulp, which was named DlMC4.The results of homologous alignment and phylogenetic tree analysis showed that DlMC4 had the highest similarity with litchi LcMC amino acid sequence.The quantitative results showed that the autolytic index of pulp increased with the increase of DlMC4 gene expression during storage at normal temperature after harvest, and the expression of DlMC4 gene in 'Dongbi' pulp with higher autolysis index was higher than that in 'Shixia' pulp with lower autolysis index.The analysis of Caspase-4 enzyme activity in pulp showed that the expression of DlMC4 gene was positively correlated with the enzyme activity and autolysis index of pulp.Therefore, the up-regulated expression of DlMC4 gene may promote autolysis of pulp in the later stage of postharvest storage.
【作者單位】： 華南農(nóng)業(yè)大學(xué)園藝學(xué)院廣東省果蔬保鮮重點(diǎn)實(shí)驗(yàn)室;賀州學(xué)院食品科學(xué)與工程技術(shù)研究院;廣東省農(nóng)業(yè)科學(xué)院果樹研究所農(nóng)業(yè)部南亞熱帶果樹生物學(xué)與遺傳資源利用重點(diǎn)實(shí)驗(yàn)室;
【基金】：國家現(xiàn)代農(nóng)業(yè)產(chǎn)業(yè)技術(shù)體系(荔枝龍眼CARS-33-14) 廣東省揚(yáng)帆計(jì)劃項(xiàng)目(No.2014YT02H013)
【分類號】：S667.2
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本文編號：1714134