本文選題：間日瘧原蟲　切入點(diǎn)：MSP-基因　出處：《中國血吸蟲病防治雜志》2017年01期

【摘要】：目的分析山東省間日瘧原蟲MSP-1和CSP基因類型及其同源性,為病例溯源提供科學(xué)依據(jù)。方法采集2011年山東省報(bào)告的12例間日瘧患者血樣,提取瘧原蟲基因組DNA;分別根據(jù)間日瘧原蟲MSP-1和CSP基因序列設(shè)計(jì)引物,進(jìn)行巢式PCR擴(kuò)增、酶切、測序、序列比對及同源性分析。結(jié)果 12份間日瘧患者血樣MSP-1基因全部出現(xiàn)470bp擴(kuò)增條帶以及350、120 bp酶切片段,均為Sal-1型;MSP-1進(jìn)化樹分析顯示,9份省內(nèi)感染者樣品序列同屬一個(gè)分枝,1份印度感染者樣品序列與印度分離株位于同一分枝。12份間日瘧患者樣品CSP基因均包含GDRA(D/A)GQPA序列,為PV-Ⅰ型,其中10份省內(nèi)感染者和1份廣東感染者樣品CSP基因出現(xiàn)560~840 bp和150~230 bp兩種擴(kuò)增條帶,為PV-Ⅰ型溫帶族,1份在印度感染者樣品CSP基因僅出現(xiàn)560~840 bp條帶,為PV-Ⅰ型熱帶族。CSP進(jìn)化樹表明,10份省內(nèi)感染者及1份廣東感染者樣品序列同屬一個(gè)分枝,1份在印度感染者樣品序列與印度和印度尼西亞分離株位于同一分枝。結(jié)論山東省本地感染間日瘧原蟲MSP-1基因型均為Sal-1型,CSP基因型均為PV-Ⅰ型溫帶族,本地蟲株具有較強(qiáng)的基因同源性。
[Abstract]:Objective to analyze the types and homology of MSP-1 and CSP genes of Plasmodium vivax in Shandong Province, and to provide scientific basis for tracing the case.Methods the genomic DNA of Plasmodium vivax was extracted from 12 cases of Plasmodium vivax reported in Shandong Province in 2011, and primers were designed according to the sequence of MSP-1 and CSP genes of Plasmodium vivax. The primers were amplified by nested PCR, digested by enzyme, sequenced, sequenced and homologous analyzed.Results all of the 12 samples of vivax malaria had 470bp amplified bands and 350120 BP restriction fragment.The results of phylogenetic tree analysis showed that the CSP gene of 9 infected individuals in the province belonged to the same branch and one Indian infection sample, and the CSP gene of 12 vivax malaria patients with vivax malaria isolates located in the same branch as the Indian isolate contained the GDRA(D/A)GQPA sequence, which belonged to PV- 鈪，

本文編號(hào)：1710251