本文選題：番茄　切入點(diǎn)：SlGH3-15　出處：《華中農(nóng)業(yè)大學(xué)》2016年碩士論文

【摘要】：番茄是一種重要的蔬菜,營養(yǎng)豐富,果實(shí)多汁,既可以鮮食又可以加工。市場上具有不同優(yōu)良性狀的番茄新品種,受到了越來越多消費(fèi)者的青睞�？刂品阎匾誀罨虻陌l(fā)掘以及功能解析對(duì)番茄遺傳育種和滿足番茄多樣化的市場需求有重要意義�，F(xiàn)代番茄是經(jīng)過野生小果的醋栗番茄到櫻桃番茄的馴化,以及隨后的栽培改良演變而來。本實(shí)驗(yàn)室前期依靠第二測序技術(shù)對(duì)300多份番茄核心種質(zhì)材料進(jìn)行了重測序,發(fā)現(xiàn)在此300多份番茄材料的基因組上有大量的核苷酸變異,這些變異為番茄由野生小果番茄到大果番茄馴化和改良過程中所涉及到受到選擇的位點(diǎn)提供了證據(jù)。通過比較分析,課題組在番茄第12染色體的受選擇區(qū)域上發(fā)現(xiàn)了一個(gè)SlGH3-15基因,根據(jù)其所在染色體的位置,推測其可能與番茄果實(shí)的發(fā)育相關(guān)。本研究通過轉(zhuǎn)基因?qū)嶒?yàn),對(duì)SlGH3-15基因的生物學(xué)功能進(jìn)行解析,得到了如下結(jié)論：1、在番茄中超量表達(dá)SlGH3-15基因,轉(zhuǎn)基因植株的形態(tài)發(fā)生顯著的變化,植株整體矮化,節(jié)間距變短,葉片卷曲,側(cè)根變少,并且在植株生長初期植株的側(cè)根的數(shù)目就比對(duì)照材料少,而且這些變化有顯著的差異；2、在番茄中超量表達(dá)SlGH3-15基因,轉(zhuǎn)基因植株體內(nèi)自由態(tài)IAA含量比對(duì)照材料減少,并且具有顯著差異；3、在番茄中干涉SlGH3-15基因表達(dá),轉(zhuǎn)基因植株形態(tài)與對(duì)照植株沒有明顯差異,此外轉(zhuǎn)基因植株體內(nèi)自由態(tài)IAA含量與對(duì)照也沒有顯著差異；4.SlGH3-15基因主要在細(xì)胞核中和細(xì)胞膜上行使功能,并且超量表達(dá)的番茄植株葉片細(xì)胞層數(shù)比對(duì)照植株少,細(xì)胞形態(tài)與對(duì)照材料也存在差異；5、外源噴施NAA能夠部分恢復(fù)超量表達(dá)SlGH3-15基因轉(zhuǎn)基因株系的表型。
[Abstract]:Tomato is an important vegetable, rich in nutrition, juicy fruit, can be both fresh and processing.New tomato varieties with different excellent characters are favored by more and more consumers on the market.The discovery of genes controlling the importance of tomato and its function analysis are of great significance to tomato genetics and breeding and to meet the market demand of tomato variety.Modern tomatoes have evolved from wild currant tomatoes to cherry tomatoes, as well as subsequent cultivation improvements.In our laboratory, we resequenced more than 300 tomato core collection materials by the second sequencing technique, and found that there were a lot of nucleotide variations in the genome of more than 300 tomato materials.These variations provide evidence for the selection of sites involved in the domestication and improvement of tomato from wild small fruit tomato to large fruit tomato.Through comparative analysis, we found a SlGH3-15 gene in the selected region of tomato chromosome 12. According to the location of the chromosome, we speculated that it might be related to the development of tomato fruit.In this study, the biological function of SlGH3-15 gene was analyzed by transgenic experiment, and the following conclusions were obtained: 1. Overexpressing SlGH3-15 gene in tomato, the morphology of transgenic plants changed significantly, the whole plant was dwarfed, and the internode spacing became shorter.Leaves curled, lateral roots became less, and the number of lateral roots in the early growth stage of the plant was less than that of the control, and there was significant difference in these changes. The SlGH3-15 gene was overexpressed in tomato.The content of free IAA in transgenic plants was lower than that of control plants, and there was significant difference between transgenic plants and control plants. There was no significant difference in the expression of SlGH3-15 gene between transgenic plants and control plants, and there was no significant difference in morphology between transgenic plants and control plants.In addition, there was no significant difference in free state IAA content between transgenic plants and control plants. 4. SlGH3-15 gene mainly functioned in nucleus and cell membrane, and the number of cell layers in leaves of transgenic plants was less than that of control plants.There was also a difference between the cell morphology and the control material. Exogenous NAA spraying could partially restore the phenotype of transgenic lines expressing SlGH3-15 gene.
【學(xué)位授予單位】：華中農(nóng)業(yè)大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2016
【分類號(hào)】：S641.2

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本文編號(hào)：1709686