發(fā)布時間：2018-04-04 07:15

  本文選題：枇杷　切入點(diǎn)：ASR基因　出處：《中國南方果樹》2017年03期

【摘要】：分離了枇杷ASR(abscisic acid,stress and ripening-induced)cDNA全長基因EjASR1,該基因長為877bp,含有1個822bp的開放閱讀框,編碼241個氨基酸。EjASR1基因編碼的蛋白質(zhì)與荔枝、甜橙親緣關(guān)系較近,與水稻等親緣關(guān)系較遠(yuǎn)。熒光定量RT-PCR分析顯示,EjASR1表達(dá)量隨著果實(shí)發(fā)育呈上升趨勢,在果實(shí)發(fā)育后期EjASR1的表達(dá)量顯著高于發(fā)育前期,表明該基因與成熟相關(guān)。
[Abstract]:The full-length gene EjASR1 of ASR(abscisic acidinstress and ripening-induced)cDNA was isolated. The gene was 877bplong and contained an open reading frame of 822bp. The protein encoding 241 amino acid. EjASR1 gene was closely related to litchi and sweet orange, and was far related to rice and so on.Fluorescence quantitative RT-PCR analysis showed that the expression of EjASR1 increased with fruit development, and the expression of EjASR1 was significantly higher in the later stage of fruit development than in the early stage of fruit development, indicating that the expression of EjASR1 was related to maturation.
【作者單位】： 浙江農(nóng)林大學(xué)農(nóng)業(yè)與食品科學(xué)學(xué)院;浙江省杭州市余杭區(qū)農(nóng)業(yè)技術(shù)推廣中心;浙江省寧�？h林特技術(shù)推廣總站;浙江省農(nóng)業(yè)科學(xué)院園藝研究所;
【基金】：浙江省寧波市農(nóng)業(yè)重大重點(diǎn)項(xiàng)目(2013C11022) 浙江省杭州市農(nóng)業(yè)豐收獎項(xiàng)目(2015F2-1) 國家自然科學(xué)基金面上項(xiàng)目(31570679) 浙江省果品新品種選育重大專項(xiàng)(2016C02052-3)資助
【分類號】：S667.3
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本文編號：1708919