本文選題：杜仲　切入點：愈傷組織　出處：《分子植物育種》2017年01期

【摘要】：通過對杜仲愈傷組織轉(zhuǎn)錄組高通量測序結(jié)果進行KEGG通路深入分析發(fā)現(xiàn),強光(光強為12 000 Lx,16 h光照,8 h黑暗)培養(yǎng)杜仲愈傷組織18 d,黑暗培養(yǎng)作為對照,與類胡蘿卜素合成相關(guān)的七種酶(九個基因)上調(diào)表達,七種酶分別為9-順式-環(huán)氧類胡蘿卜素雙加氧酶(EC 1.13.11.51,9-cis-epoxycarotenoid dioxygenase),脫落酸8-羥化酶(EC 1.14.13.93,(+)-abscisic acid 8'-hydroxylase),紫黃質(zhì)脫環(huán)氧化酶(EC 1.10.99.3,violaxanthin de-epoxidase),15-順式-八氫番茄紅素合成酶(EC 2.5.1.32,15-cis-phytoene synthase),番茄紅素環(huán)化酶(Cru A),β-胡蘿卜素羥化酶(Crt R-b),八氫番茄紅素脫飽和酶(Crtl-e)。qRT-PCR分析表明,杜仲愈傷組織中與類胡蘿卜素合成相關(guān)的九個基因的表達與其轉(zhuǎn)錄組高通量測序結(jié)果一致,均上調(diào)表達。由此推斷,強光促進杜仲愈傷組織中類胡蘿卜素的積累。本研究可以為獲取高產(chǎn)類胡蘿卜素資源和杜仲遺傳分析提供有價值的資料。
[Abstract]:The high throughput sequencing of eucommia ulmoides callus transcriptional group was analyzed by KEGG pathway. It was found that the callus of Eucommia ulmoides was cultured under intense light (light intensity 12 000 L x ~ (16) h) and dark light for 8 h for 18 days, and dark culture was used as control.Seven enzymes (nine genes) associated with carotenoid synthesis upregulated expression.涓冪閰跺垎鍒負9-欏哄紡-鐜哀綾昏儭钀濆崪绱犲弻鍔犳哀閰，

本文編號：1707954