本文選題：金針菇　切入點(diǎn)：子實(shí)體　出處：《食用菌學(xué)報(bào)》2017年03期

【摘要】：通過分析金針菇(Flammulina velutipes)子實(shí)體原基期、伸長期、成熟期的轉(zhuǎn)錄組數(shù)據(jù),得到一個(gè)差異表達(dá)基因fv-msf1。對其結(jié)構(gòu)、序列特征及表達(dá)情況進(jìn)行分析,結(jié)果顯示該基因全長2709 bp,包含13個(gè)外顯子,12個(gè)內(nèi)含子,開放閱讀框長1770 bp,編碼589個(gè)氨基酸。結(jié)構(gòu)域分析表明該基因編碼的蛋白含主要協(xié)助轉(zhuǎn)運(yùn)蛋白超家族(major facilitator superfamily,MFS)保守結(jié)構(gòu)域。通過實(shí)時(shí)熒光定量PCR(real-time quantitative fluorescence PCR,q-PCR)對子實(shí)體不同發(fā)育階段以及菌蓋不同發(fā)育時(shí)期的表達(dá)量進(jìn)行分析,結(jié)果顯示該基因在金針菇伸長期的菌蓋(尤其是0.3~0.9cm)中高表達(dá),由此推斷該基因在金針菇菌蓋發(fā)育中發(fā)揮一定作用。
[Abstract]:A differentially expressed gene fv-msf1 was obtained by analyzing the transcriptional data of Flammulina velutipesae during the primary, extension and maturation stages of the fruiting body of Flammulina velutipesae (Flammulina velutipesae).The results showed that the gene was 2709 BP in length, including 13 exons and 12 introns, and the open reading frame was 1770 BP long, encoding 589 amino acids.Domain analysis showed that the protein encoded by the gene contained major facilitator superfamily (MFS) conserved domain.The expression of Flammulina velutipes (Flammulina velutipes) in different developmental stages of fruiting bodies and different developmental stages of capsid was analyzed by real-time PCR(real-time quantitative fluorescence PCRq-PCRQ. The results showed that the gene was highly expressed in the lid of Flammulina velutipes during the extension period, especially 0.30.9cmof Flammulina velutipes (Flammulina velutipes).It is concluded that this gene plays a certain role in the development of Flammulina velutipes (Flammulina velutipes).
【作者單位】： 福建農(nóng)林大學(xué)生命科學(xué)學(xué)院;福建農(nóng)林大學(xué)園藝學(xué)院;
【基金】：國家重點(diǎn)基礎(chǔ)研究發(fā)展計(jì)劃(2014CB138302) 福建省現(xiàn)代農(nóng)業(yè)食用菌產(chǎn)業(yè)技術(shù)體系項(xiàng)目(K83139295)資助
【分類號(hào)】：S646.15
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本文編號(hào)：1701041