本文選題：玉米大斑病菌　切入點：StSte基因　出處：《河南農(nóng)業(yè)科學》2016年01期

【摘要】：以葡萄糖、蔗糖、乳糖和可溶性淀粉為碳源分別培養(yǎng)玉米大斑病菌野生型和St Ste12基因RNAi陽性轉(zhuǎn)化子,研究碳源對St Ste12基因調(diào)控玉米大斑病菌菌絲生長和分生孢子發(fā)育的影響,為闡明St Ste12基因的功能和病菌的致病機制奠定基礎。結(jié)果表明,St Ste12基因?qū)τ衩状蟀卟【z生長和分生孢子發(fā)育均有重要的調(diào)控作用,St Ste12基因的表達量下降后,菌絲的生長勢變?nèi)?顏色變淺,生長速度顯著下降,產(chǎn)孢量顯著降低。4種供試碳源中,可溶性淀粉對St Ste12基因調(diào)控菌絲生長和發(fā)育的影響最大,蔗糖最小;乳糖對St Ste12基因調(diào)控分生孢子發(fā)育沒有顯著影響,而其他3種碳源的影響均達到顯著水平。
[Abstract]:Glucose, sucrose, lactose and soluble starch were used as carbon sources to culture wild type and St Ste12 gene RNAi positive transformants of maize spotted spot fungus. The effects of carbon source on the regulation of mycelium growth and conidial development by St Ste12 gene were studied. In order to clarify the function of St Ste12 gene and the pathogenic mechanism of the pathogen, the results showed that St Ste12 gene had an important role in regulating mycelium growth and conidial development of Corn spotted spot, and the expression of St Ste12 gene was decreased after the decrease of the expression of St Ste12 gene. The growth potential of mycelium became weaker, the color became lighter, the growth rate decreased significantly, and the spore production decreased significantly. Among the 4 carbon sources tested, soluble starch had the greatest effect on the regulation of hyphal growth and development by St Ste12 gene, and sucrose was the smallest. Lactose had no significant effect on the conidial development of St Ste12 gene, but the effect of the other three carbon sources was significant.
【作者單位】： 唐山師范學院生命科學系;
【基金】：國家自然科學基金項目(31271997) 河北省自然科學基金項目(C2014105067) 唐山師范學院團隊建設基金項目(2014E04)
【分類號】：S435.131

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