本文選題：玉米大斑病菌　切入點(diǎn)：StSte基因　出處：《河南農(nóng)業(yè)科學(xué)》2016年01期

【摘要】：以葡萄糖、蔗糖、乳糖和可溶性淀粉為碳源分別培養(yǎng)玉米大斑病菌野生型和St Ste12基因RNAi陽(yáng)性轉(zhuǎn)化子,研究碳源對(duì)St Ste12基因調(diào)控玉米大斑病菌菌絲生長(zhǎng)和分生孢子發(fā)育的影響,為闡明St Ste12基因的功能和病菌的致病機(jī)制奠定基礎(chǔ)。結(jié)果表明,St Ste12基因?qū)τ衩状蟀卟【z生長(zhǎng)和分生孢子發(fā)育均有重要的調(diào)控作用,St Ste12基因的表達(dá)量下降后,菌絲的生長(zhǎng)勢(shì)變?nèi)?顏色變淺,生長(zhǎng)速度顯著下降,產(chǎn)孢量顯著降低。4種供試碳源中,可溶性淀粉對(duì)St Ste12基因調(diào)控菌絲生長(zhǎng)和發(fā)育的影響最大,蔗糖最小;乳糖對(duì)St Ste12基因調(diào)控分生孢子發(fā)育沒有顯著影響,而其他3種碳源的影響均達(dá)到顯著水平。
[Abstract]:Glucose, sucrose, lactose and soluble starch were used as carbon sources to culture wild type and St Ste12 gene RNAi positive transformants of maize spotted spot fungus. The effects of carbon source on the regulation of mycelium growth and conidial development by St Ste12 gene were studied. In order to clarify the function of St Ste12 gene and the pathogenic mechanism of the pathogen, the results showed that St Ste12 gene had an important role in regulating mycelium growth and conidial development of Corn spotted spot, and the expression of St Ste12 gene was decreased after the decrease of the expression of St Ste12 gene. The growth potential of mycelium became weaker, the color became lighter, the growth rate decreased significantly, and the spore production decreased significantly. Among the 4 carbon sources tested, soluble starch had the greatest effect on the regulation of hyphal growth and development by St Ste12 gene, and sucrose was the smallest. Lactose had no significant effect on the conidial development of St Ste12 gene, but the effect of the other three carbon sources was significant.
【作者單位】： 唐山師范學(xué)院生命科學(xué)系;
【基金】：國(guó)家自然科學(xué)基金項(xiàng)目(31271997) 河北省自然科學(xué)基金項(xiàng)目(C2014105067) 唐山師范學(xué)院團(tuán)隊(duì)建設(shè)基金項(xiàng)目(2014E04)
【分類號(hào)】：S435.131

【相似文獻(xiàn)】

相關(guān)期刊論文 前10條

1 劉振盼;王艷輝;董金皋;;玉米大斑病菌完整染色體DNA的制備方法[J];華北農(nóng)學(xué)報(bào);2007年06期

2 申s，

本文編號(hào)：1676618