本文選題：子宮內(nèi)膜　切入點：蛻膜化　出處：《重慶醫(yī)科大學學報》2017年08期

【摘要】：目的:檢測Nme2(nucleoside diphosphate kinase 2)基因在小鼠早期妊娠和人工誘導蛻膜化的子宮內(nèi)膜組織中的表達規(guī)律;探究Nme2基因與子宮基質(zhì)細胞蛻膜化的相關(guān)性。方法:利用免疫組織化學、Western blot和實時熒光定量PCR(q RTPCR)分別檢測小鼠妊娠第5天著床點(D5IS)、著床旁(D5IIS),D6IS、D6IIS、D8IS、D8IIS及人工誘導蛻膜化模型小鼠子宮內(nèi)膜中Nme2的表達定位,m RNA和蛋白質(zhì)水平的表達水平。結(jié)果:小鼠D5、D6、D8子宮內(nèi)膜中著床點Nme2基因的m RNA和蛋白水平的表達明顯高于著床旁;免疫組織化學結(jié)果顯示Nme2表達定位表達于D5著床點的初級蛻膜區(qū)(primary decidua region,PDZ)、D6和D8著床點的次級蛻膜區(qū)(secondary decidua region,SDZ);人工誘導蛻膜化模型中,Nme2基因的m RNA和蛋白質(zhì)水平在誘導組的表達明顯高于對照組。結(jié)論:Nme2基因的表達上調(diào)與小鼠基質(zhì)細胞的蛻膜化過程相關(guān)。
[Abstract]:Objective: to investigate the expression of Nme2(nucleoside diphosphate kinase 2 (Nme2(nucleoside diphosphate kinase 2) gene in early pregnancy and decidualized endometrium of mice. To explore the relationship between Nme2 gene and decidualization of uterine stromal cells. Methods: immunohistochemical blot and real time fluorescence quantitative PCR(q were used to detect D5ISN, D5IISN, D6ISD6IIS-D8ISD8IIS and artificial induced decidualization model in mice on the 5th day of pregnancy, respectively. Results: the expression of m RNA and protein of Nme2 gene at implantation site was significantly higher in mouse endometrium than that near implantation site. Immunohistochemical results showed that the expression of Nme2 was localized in primary decidua decidua region of D5 implantation site and secondary decidua region of D8 implantation site, and m RNA and protein levels of Nme2 gene were induced in artificial decidualization model. Conclusion the upregulation of the expression of the 1: Nme2 gene is related to the process of decidualization of mouse stromal cells.
【作者單位】： 重慶醫(yī)科大學公共衛(wèi)生與管理學院生殖生物學研究室;重慶醫(yī)科大學中醫(yī)藥學院中藥綜合教研室;
【基金】：國家自然科學基金資助項目(編號:81671493)
【分類號】：R714

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