本文選題：Atlantic　切入點：無標記基因　出處：《分子植物育種》2017年11期

【摘要】：本研究以加工型專用品種Atlantic為實驗材料,分別對影響其遺傳轉化的外植體、激素、預培養(yǎng)、菌液濃度、侵染及共培養(yǎng)等因素進行優(yōu)化,建立了農桿菌介導的無標記基因高效轉化體系,初步獲得了轉基因植株。研究結果表明,葉片和莖段均可作為馬鈴薯品種Atlantic的受體材料,轉化過程中以預培養(yǎng)2~3 d后用OD600=0.5的菌液侵染10~15 min,在MSGⅠ(添加有5 mg/L NAA和1.0 mg/L 6-BA)培養(yǎng)基上共培養(yǎng)2~3d后,轉入含有250 mg/L的MSGⅡ(0.02 mg/L GA3和2.0 g/L ZT)的培養(yǎng)基上誘導生芽的遺傳轉化效率最高,其愈傷誘導率可達83.34%,芽分化率可達48.3%。通過該轉化體系將抗馬鈴薯PVY病毒的基因導入馬鈴薯,獲得無標記基因的轉化植株,經(jīng)PCR檢測,初步確定已經(jīng)將外源目的基因導入了馬鈴薯基因組中。
[Abstract]:In this study, the explant, hormone, preculture, bacterial liquid concentration, infection and co-culture were optimized with Atlantic as the experimental material. Agrobacterium tumefaciens mediated high efficiency transformation system of unlabeled genes was established and transgenic plants were obtained. The results showed that both leaf and stem segments could be used as receptor materials for potato variety Atlantic. During the transformation, the cells were precultured for 2 days, then infected with OD600=0.5 solution for 1015 min, then co-cultured on the medium of MSG 鈪，

本文編號：1662008