本文選題：豬流行性腹瀉病毒　切入點：變異株　出處：《內(nèi)蒙古大學學報(自然科學版)》2017年02期

【摘要】：為分析山東省2016年上半年豬流行性腹瀉病毒(PEDV)流行株變異情況,用Vero細胞從仔豬臨床腹瀉樣品中進行病毒分離,通過細胞病變(CPE)觀察、聚合酶鏈式反應(PCR)方法、免疫熒光(IFA)試驗、電鏡觀察、動物回歸試驗和測序分析,分離鑒定1株P(guān)EDV毒株(SDMY1401株).將該毒株進行S基因序列分析和遺傳進化分析,結(jié)果表明該毒株為PEDV流行變異株.該毒株S基因核苷酸序列與山東省分離株CH-SDZC-2015和河南省分離株CHHNAY-2015同源性最高為99.4%,與疫苗毒株CV777同源性為94%.遺傳進化分析表明該毒株與經(jīng)典毒株DR13、疫苗毒株CV777及以前國內(nèi)分離毒株在不同分支.
[Abstract]:In order to analyze the variation of epidemic strains of porcine epidemic diarrhea virus (PEVV) in Shandong Province in the first half of 2016, Vero cells were isolated from clinical diarrhea samples of piglets, observed by cytopathic acid (CPE) and polymerase chain reaction (PCR) method. A strain of PEDV strain SDMY1401 was isolated and identified by immunofluorescence assay, electron microscope observation, animal regression test and sequencing analysis. The S gene sequence and genetic evolution of the strain were analyzed. The results showed that the nucleotide sequence of S gene of this strain was the most homologous to CH-SDZC-2015 of Shandong Province and CHHNAY-2015 of Henan Province, and 94% to CV777 of vaccine strain. Genetic evolution analysis showed that this strain had the highest homology with CH-SDZC-2015 strain of Shandong Province and CHHNAY-2015 of Henan Province strain. The genetic evolution analysis showed that this strain had the highest homology with the vaccine strain CV777. Strain DR13, vaccine strain CV777 and previously isolated strains were in different branches.
【作者單位】： 青島農(nóng)業(yè)大學動物科技學院;山東省畜禽疫病防治與繁育重點實驗室;山東省農(nóng)業(yè)科學院畜牧獸醫(yī)研究所;
【基金】：泰山學者工程經(jīng)費資助 山東省現(xiàn)代農(nóng)業(yè)產(chǎn)業(yè)技術(shù)體系(SDAIT) 山東省農(nóng)業(yè)重大應用技術(shù)創(chuàng)新項目 山東省農(nóng)業(yè)科學院農(nóng)業(yè)科技創(chuàng)新工程(CXGC2016B14);山東省農(nóng)業(yè)科學院重大科技成果培育計劃(2014CGPY04)
【分類號】：S852.65

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