發(fā)布時間：2018-03-22 13:02

  本文選題：CryBb毒素　切入點：轉(zhuǎn)基因植物　出處：《江蘇農(nóng)業(yè)學報》2017年03期 　論文類型：期刊論文

【摘要】：本研究利用競爭洗脫法淘選與Cry3Bb毒素具有親和力的特異性短肽,以此建立間接競爭酶聯(lián)免疫吸附檢測方法(IC-ELISA)。經(jīng)過4輪淘選、富集,獲得與靶蛋白具有較高親和力的B10克隆(ACIHSPTALCGGG)。所建立的檢測方法穩(wěn)定性較好,變異系數(shù)在5%以內(nèi);線性檢測范圍為0.03~29.95μg/ml,線性回歸方程為(Y=20.044x+50.407,R2=0.983 4),檢測限為0.009 6μg/ml,在玉米樣品中提取回收率為92.26%~101.22%。本研究所建立的ELISA檢測方法為糧食中Cry3Bb蛋白的定量檢測提供了有效的手段,在轉(zhuǎn)基因產(chǎn)品的檢驗檢疫中有較高的應用價值。
[Abstract]:In this study, an indirect competitive enzyme linked immunosorbent assay (IC-ELISAA) was established for the detection of short peptides with affinity to Cry3Bb toxin by competitive elution method. B10 clone with high affinity to target protein was obtained. The stability of the method was good, and the coefficient of variation was less than 5%. The linear detection range was 0.03 渭 g / ml, the linear regression equation was 20.044x 50.407R20.9834, the detection limit was 0.009 渭 g / ml, and the recovery rate was 92.26g / ml. The ELISA method established in this study provided an effective method for the quantitative detection of Cry3Bb protein in grain. It has high application value in the inspection and quarantine of transgenic products.
【作者單位】： 金陵科技學院園藝學院;江蘇省農(nóng)業(yè)科學院食品質(zhì)量安全與檢測研究所/農(nóng)業(yè)部食品質(zhì)量安全監(jiān)控重點開放實驗室;
【基金】：江蘇省自然科學基金青年基金項目(BK20150114) 江蘇省高校自然科學研究項目(14KJB 210004) 金陵科技學院博士啟動項目(jit-6-201518);金陵科技學院校級科研基金孵化項目(C140501)
【分類號】：S188
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本文編號：1648761