本文選題：前列腺癌　切入點：miR-　出處：《實用醫(yī)學雜志》2017年22期 　論文類型：期刊論文

【摘要】：目的研究miR-195靶向抑制S6K1基因調控前列腺癌細胞增殖、凋亡、侵襲和轉移活性。方法選擇2種前列腺癌細胞株DU145和LNCaP,實驗分為3組,即空白對照組、過表達組和低表達組,構建DU145和LNCaP過表達和沉默表達miR-195的細胞株,分別培養(yǎng)24 h,采用反轉錄PCR(RT-PCR)法鑒定轉染效果,MTT法檢測細胞增殖率,流式細胞術檢測凋亡率,Transwell侵襲實驗和細胞劃痕實驗檢測細胞侵襲和轉移能力;RT-PCR法檢測S6K1 mRNA相對表達水平,熒光素酶報告系統(tǒng)確認miR-195直接調控的靶基因。結果過表達組細胞增殖率顯著低于對照組,低表達組最高;凋亡率高于對照組,低表達組最低;侵襲細胞數目和劃痕距離小于對照組,低表達組最大;S6K1 mRNA相對表達水平低于對照組,低表達組最高。S6K1基因為miR-195的靶基因。結論 miR-195靶向抑制S6K1基因調控前列腺癌細胞的增殖、凋亡、侵襲和轉移活性。
[Abstract]:Objective to study the inhibitory effect of miR-195 on the proliferation, apoptosis, invasion and metastasis of prostate cancer cell line DU145 and LNCaP.Methods two kinds of prostate cancer cell lines DU145 and LNCaP were divided into three groups: blank control group, overexpression group and low expression group. DU145 and LNCaP overexpression and silencing expression of miR-195 were constructed and cultured for 24 h respectively. The transfection effect was evaluated by reverse transcription PCR RT-PCR assay. The apoptosis rate was detected by flow cytometry and the relative expression of S6K1 mRNA was detected by reverse transcription-polymerase chain reaction (RT-PCR). Luciferase reporting system confirmed the target gene directly regulated by miR-195. Results the cell proliferation rate of overexpression group was significantly lower than that of control group, the highest in low expression group, the apoptosis rate was higher than that in control group, and the lowest in low expression group. The relative expression level of S6K1 mRNA in the low expression group was lower than that in the control group, and the highest S6K1 gene in the low expression group was due to the target gene of miR-195. Conclusion miR-195 can inhibit the proliferation of prostate cancer cells regulated by S6K1 gene. Apoptosis, invasion and metastasis activity.
【作者單位】： 南華大學附屬第二醫(yī)院泌尿外科;南華大學附屬南華醫(yī)院麻醉科;
【基金】：湖南省衡陽市科技計劃項目(編號:2016KJ32)
【分類號】：R737.25
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本文編號：1647428