本文選題：前列腺癌　切入點(diǎn)：miR-　出處：《實(shí)用醫(yī)學(xué)雜志》2017年22期 　論文類型：期刊論文

【摘要】：目的研究miR-195靶向抑制S6K1基因調(diào)控前列腺癌細(xì)胞增殖、凋亡、侵襲和轉(zhuǎn)移活性。方法選擇2種前列腺癌細(xì)胞株DU145和LNCaP,實(shí)驗(yàn)分為3組,即空白對(duì)照組、過表達(dá)組和低表達(dá)組,構(gòu)建DU145和LNCaP過表達(dá)和沉默表達(dá)miR-195的細(xì)胞株,分別培養(yǎng)24 h,采用反轉(zhuǎn)錄PCR(RT-PCR)法鑒定轉(zhuǎn)染效果,MTT法檢測(cè)細(xì)胞增殖率,流式細(xì)胞術(shù)檢測(cè)凋亡率,Transwell侵襲實(shí)驗(yàn)和細(xì)胞劃痕實(shí)驗(yàn)檢測(cè)細(xì)胞侵襲和轉(zhuǎn)移能力;RT-PCR法檢測(cè)S6K1 mRNA相對(duì)表達(dá)水平,熒光素酶報(bào)告系統(tǒng)確認(rèn)miR-195直接調(diào)控的靶基因。結(jié)果過表達(dá)組細(xì)胞增殖率顯著低于對(duì)照組,低表達(dá)組最高;凋亡率高于對(duì)照組,低表達(dá)組最低;侵襲細(xì)胞數(shù)目和劃痕距離小于對(duì)照組,低表達(dá)組最大;S6K1 mRNA相對(duì)表達(dá)水平低于對(duì)照組,低表達(dá)組最高。S6K1基因?yàn)閙iR-195的靶基因。結(jié)論 miR-195靶向抑制S6K1基因調(diào)控前列腺癌細(xì)胞的增殖、凋亡、侵襲和轉(zhuǎn)移活性。
[Abstract]:Objective to study the inhibitory effect of miR-195 on the proliferation, apoptosis, invasion and metastasis of prostate cancer cell line DU145 and LNCaP.Methods two kinds of prostate cancer cell lines DU145 and LNCaP were divided into three groups: blank control group, overexpression group and low expression group. DU145 and LNCaP overexpression and silencing expression of miR-195 were constructed and cultured for 24 h respectively. The transfection effect was evaluated by reverse transcription PCR RT-PCR assay. The apoptosis rate was detected by flow cytometry and the relative expression of S6K1 mRNA was detected by reverse transcription-polymerase chain reaction (RT-PCR). Luciferase reporting system confirmed the target gene directly regulated by miR-195. Results the cell proliferation rate of overexpression group was significantly lower than that of control group, the highest in low expression group, the apoptosis rate was higher than that in control group, and the lowest in low expression group. The relative expression level of S6K1 mRNA in the low expression group was lower than that in the control group, and the highest S6K1 gene in the low expression group was due to the target gene of miR-195. Conclusion miR-195 can inhibit the proliferation of prostate cancer cells regulated by S6K1 gene. Apoptosis, invasion and metastasis activity.
【作者單位】： 南華大學(xué)附屬第二醫(yī)院泌尿外科;南華大學(xué)附屬南華醫(yī)院麻醉科;
【基金】：湖南省衡陽(yáng)市科技計(jì)劃項(xiàng)目(編號(hào):2016KJ32)
【分類號(hào)】：R737.25
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本文編號(hào)：1647428