本文選題：CD　切入點：基因多態(tài)性　出處：《中國老年學(xué)雜志》2017年01期 　論文類型：期刊論文

【摘要】：目的探討動脈硬化心臟病與CD36單核苷酸多態(tài)性的關(guān)系。方法 121例動脈硬化心臟病患者及體檢的健康者217例進(jìn)行研究;空腹12 h后采集靜脈血5 ml,檢查甘油三酯、空腹血糖、血細(xì)胞、低密度脂蛋白、總膽固醇、高密度脂蛋白、血栓素B2等主要生化指標(biāo),記錄其體質(zhì)量、升高、體質(zhì)量指數(shù)、血壓等一般指標(biāo),并詳細(xì)問詢其疾病史;采用聚合酶鏈反應(yīng)-連接酶檢測反應(yīng)法(PCR-LDR法)檢測CD36的SNPs基因型。結(jié)果對照組冠心病、高血壓、糖尿病患者比例及血壓、體質(zhì)量指數(shù)、甘油三酯、血糖、總膽固醇、血栓素、低密度脂蛋白顯著低于病例組(P0.05),病例組患者血高密度脂蛋白水平顯著低于對照組(P0.05);rs1761667位點存GG、AG、AA 3種基因型,兩組基因頻率差異具統(tǒng)計學(xué)意義(P0.05);分析分布頻率發(fā)現(xiàn),病例組AG基因型顯著高于對照組(P0.05);AG基因型人群中病例組患者收縮壓、舒張壓、體質(zhì)量指數(shù)、總膽固醇、甘油三酯、低密度脂蛋白顯著高于對照組(P0.05),高密度脂蛋白水平顯著低于對照組(P0.05);AG基因型均可明顯增加動脈硬化心臟病的患病風(fēng)險(P0.05);甘油三酯、收縮壓、糖尿病史、冠心病史、低密度脂蛋白與rs1761667AG基因型為影響動脈硬化心臟病的獨立性危險因素(P0.05);CD36 SNPs具有群體代表性,符合Hardy-Weinberg平衡定律(P0.05)。結(jié)論動脈硬化心臟病與CD36 rs1761667的AG型存相關(guān)關(guān)系,是導(dǎo)致動脈硬化心臟病的遺傳因素。
[Abstract]:Objective to investigate the relationship between single nucleotide polymorphism (SNP) of CD36 and arteriosclerotic heart disease (AHD). Methods 121 patients with arteriosclerotic heart disease and 217 healthy subjects were studied, 5 ml venous blood samples were collected 12 hours after fasting, triglyceride and fasting blood glucose were measured. The main biochemical indexes, such as blood cells, low density lipoprotein, total cholesterol, high density lipoprotein and thromboxane B2, were recorded and their body weight, elevation, body mass index and blood pressure were recorded. The SNPs genotypes of CD36 were detected by polymerase chain reaction-ligase assay (PCR-LDR). Results the proportion of patients with coronary heart disease, hypertension, diabetes mellitus, blood pressure, body mass index, triglyceride, blood glucose, total cholesterol, thromboxane were detected in the control group. The low density lipoprotein (LDL) was significantly lower than that in the case group (P 0.05), and the serum high density lipoprotein (HDL) level in the case group was significantly lower than that in the control group (P 0.05), rs1761667, there were three genotypes of GGG AGN AA, the difference of gene frequency between the two groups was statistically significant (P 0.05), and the distribution frequency was found. The AG genotype in the case group was significantly higher than that in the control group, including systolic blood pressure (SBP), diastolic blood pressure (DBP), body mass index (BMI), total cholesterol (TC) and triglyceride (TG). Low density lipoprotein (LDL) was significantly higher than control group (P 0.05N), high density lipoprotein (HDL) level was significantly lower than that of control group (P 0.05) AG genotype, triglyceride, systolic blood pressure (SBP), history of diabetes, history of coronary heart disease (CHD), serum triglyceride (TG), systolic blood pressure (SBP), history of diabetes, history of coronary heart disease, Low density lipoprotein (LDL) and rs1761667AG genotype were independent risk factors for atherosclerosis heart disease. P0.05 + CD36 SNPs was a group representative, which was in accordance with the Hardy-Weinberg equilibrium law (P0.05). Conclusion there is a correlation between CD36 rs1761667 AG type and atherosclerotic heart disease. Is the genetic factor that causes arteriosclerosis heart disease.
【作者單位】： 濱州醫(yī)學(xué)院附屬醫(yī)院;
【基金】：山東省自然科學(xué)基金(ZR2014HP024)
【分類號】：R541.4

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