本文選題：干細(xì)胞　切入點(diǎn)：間質(zhì)干細(xì)胞　出處：《中國(guó)組織工程研究》2017年25期 　論文類型：期刊論文

【摘要】：背景:研究發(fā)現(xiàn)基因治療和細(xì)胞移植治療視網(wǎng)膜色素變性有效果,而且二者相結(jié)合治療視網(wǎng)膜色素變性效果更為顯著,但其作用機(jī)制有待深入研究。目的:探討生長(zhǎng)相關(guān)蛋白43基因修飾脂肪間充質(zhì)干細(xì)胞對(duì)視網(wǎng)膜色素變性大鼠視網(wǎng)膜的保護(hù)作用。方法:分離和培養(yǎng)視網(wǎng)膜色素變性大鼠脂肪間充質(zhì)干細(xì)胞,以生長(zhǎng)相關(guān)蛋白43慢病毒載體轉(zhuǎn)染脂肪間充質(zhì)干細(xì)胞。將60只視網(wǎng)膜色素變性大鼠分3組干預(yù),實(shí)驗(yàn)組視網(wǎng)膜下腔注射生長(zhǎng)相關(guān)蛋白43慢病毒載體轉(zhuǎn)染的脂肪間充質(zhì)干細(xì)胞,對(duì)照組注射脂肪間充質(zhì)干細(xì)胞,假手術(shù)組注射PBS,注射后3,7,14,21 d,觀察眼球組織脂肪間充質(zhì)干細(xì)胞變化及視網(wǎng)膜生長(zhǎng)相關(guān)蛋白43表達(dá);注射后28 d,觀察視網(wǎng)膜Rho蛋白、GS蛋白表達(dá)及外核層厚度。結(jié)果與結(jié)論:(1)實(shí)驗(yàn)組脂肪間充質(zhì)干細(xì)胞移植后,隨著時(shí)間的延長(zhǎng),生長(zhǎng)相關(guān)蛋白43表達(dá)量逐漸升高,不同時(shí)間點(diǎn)間兩兩比較差異均有顯著性意義(P0.05);(2)實(shí)驗(yàn)組、對(duì)照組Rho蛋白和GS蛋白表達(dá)量高于假手術(shù)組(P0.05);實(shí)驗(yàn)組Rho蛋白表達(dá)量高于對(duì)照組(P0.05),GS蛋白表達(dá)量低于對(duì)照組(P0.05);(3)實(shí)驗(yàn)組、對(duì)照組視網(wǎng)膜外核層厚度高于假手術(shù)組(P0.05),實(shí)驗(yàn)組視網(wǎng)膜外核層厚度高于對(duì)照組(P0.05);(4)結(jié)果表明,生長(zhǎng)相關(guān)蛋白43基因修飾的脂肪間充質(zhì)干細(xì)胞,對(duì)視網(wǎng)膜色素變性大鼠模型視網(wǎng)膜具有保護(hù)作用。
[Abstract]:Background: gene therapy and cell transplantation have been found to be effective in the treatment of retinitis pigmentosa, and the combination of gene therapy and cell transplantation is more effective in the treatment of retinitis pigmentosa. Objective: to investigate the protective effect of growth associated protein 43 gene modified adipose mesenchymal stem cells on the retina of rats with retinitis pigmentosa. Methods: retinal pigmentation was isolated and cultured. Rat adipose mesenchymal stem cells, Adipose mesenchymal stem cells were transfected with growth associated protein 43 lentivirus vector. 60 rats with retinitis pigmentosa were divided into 3 groups. The experimental group was injected into the subretinal cavity of adipose mesenchymal stem cells transfected with growth associated protein 43 lentivirus vector. Adipose mesenchymal stem cells were injected into the control group and PBSs were injected into the sham operation group. The changes of adipose mesenchymal stem cells and the expression of retinal growth-associated protein 43 were observed at 21 days after injection. At 28 days after injection, the expression of Rho protein GS protein and the thickness of outer nuclear layer were observed. Results and conclusion the expression of growth-associated protein 43 increased with time after transplantation of adipose mesenchymal stem cells in the experimental group. The expression of Rho protein and GS protein in the control group was higher than that in the sham operation group, and the expression of Rho protein in the experimental group was higher than that in the control group. The thickness of the outer nuclear layer in the control group was higher than that in the sham operation group (P 0.05), and the thickness of the outer nuclear layer in the experimental group was higher than that in the control group (P 0.05). The results showed that the growth associated protein 43 gene modified adipose mesenchymal stem cells. It has protective effect on retina of rat model of retinal pigmentosa.
【作者單位】： 長(zhǎng)治醫(yī)學(xué)院解剖教研室;長(zhǎng)治醫(yī)學(xué)院附屬和平醫(yī)院;長(zhǎng)治醫(yī)學(xué)院生化教研室;
【基金】：2016年度山西省高等學(xué)�？萍紕�(chuàng)新項(xiàng)目(2016175) 長(zhǎng)治醫(yī)學(xué)院校級(jí)創(chuàng)新團(tuán)隊(duì)支持項(xiàng)目(CY201414)~~
【分類號(hào)】：R774.1

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