我國小反芻獸疫病毒H基因序列分析
發(fā)布時間:2018-03-17 20:12
本文選題:小反芻獸疫 切入點:H基因 出處:《江蘇農(nóng)業(yè)科學(xué)》2017年07期 論文類型:期刊論文
【摘要】:為了分析我國小反芻獸疫疫情的特點,從GenBank下載我國和其他國家小反芻獸疫病毒(PPRV)毒株H基因全序列,應(yīng)用分子生物學(xué)軟件DNAStar進行序列分析。結(jié)果顯示,始于2007年的我國首次PPR疫情中,我國西藏地區(qū)PPRV各毒株間H基因核苷酸同源性介于99.6%~100.0%之間,我國西藏與其他國家PPRV毒株核苷酸同源性介于86.7%~98.3%之間;我國西藏地區(qū)PPRV毒株間氨基酸序列同源性介于99.7%~100.0%之間,我國西藏與其他國家PPRV毒株氨基酸序列同源性介于89.3%~98.4%之間。始于2013年年底的我國又一次PPR疫情中,我國PPRV毒株間核苷酸同源性介于99.7%~99.8%之間,我國與其他國家PPRV毒株核苷酸同源性介于87.2%~97.5%之間;我國PPRV毒株間氨基酸序列同源性介于99.2%~99.8%之間,我國與其他國家PPRV毒株氨基酸序列同源性介于89.0%~98.4%之間。H基因進化樹分析結(jié)果顯示,我國的9株P(guān)PRV劃分為基因Ⅳ系。我國PPRV毒株與印度毒株及土耳其毒株同源性最高,可能由這2個國家傳入我國。
[Abstract]:In order to analyze the characteristics of the epidemic situation of small ruminant in China, the whole H gene sequence was downloaded from GenBank in our country and other countries, and the sequence analysis was carried out by using the molecular biology software DNAStar. In the first PPR epidemic in China, which began in 2007, the nucleotide homology of H gene among PPRV strains in Tibet was between 99.6% and 100.0%, and the nucleotide homology of PPRV strains in Tibet and other countries was between 86.7% and 98.3%. The homology of amino acid sequence among PPRV strains in Tibet region was between 99.70.0% and 98.4% between Tibet and other countries. The homology of amino acid sequence of PPRV virus strains in Tibet and other countries was between 89.3% and 98.4%. The sequence homology of amino acid sequence of PPRV virus strains in China began at the end of 2013 in another PPR epidemic in China. The nucleotide homology of PPRV strains in China was between 99.7% and 99.8%, the nucleotide homology of PPRV strains in China and other countries was between 87.2% and 97.5%, and the amino acid sequence homology of PPRV strains in China was between 99.2% and 99.8%. The amino acid sequence homology of PPRV strains in China and other countries was between 89.0% and 98.4%. The result of phylogenetic tree analysis showed that 9 PPRV strains in China were classified into gene 鈪,
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