本文選題：Notch4基因　切入點：飼鴿者肺　出處：《石河子大學(xué)》2017年碩士論文　論文類型：學(xué)位論文

【摘要】：目的:通過檢測維吾爾族飼鴿者肺外周血Notch4基因甲基化程度探討其臨床意義。方法:根據(jù)納入及排除標(biāo)準連續(xù)收集2015年-2016年在新疆喀什病例資料及血樣標(biāo)本(總納入60例,其中20例確診維吾爾族飼鴿者肺患者為病例組,同期20例維吾爾族飼養(yǎng)鴿子未發(fā)病者為陰性對照組,同期20例維吾爾族未飼養(yǎng)鴿子健康體檢者為正常對照組)。對所收集血樣標(biāo)本進行常規(guī)提取DNA,亞硫酸氫鹽轉(zhuǎn)化,PCR擴增,體外轉(zhuǎn)錄和RNase A特異性酶切,并行基質(zhì)輔助激光解吸附電離飛行時間質(zhì)譜分析技術(shù)檢測Notch4基因甲基化情況。結(jié)果:檢測Notch4片段的CpG位點總數(shù)6個(CpG_1,CpG_2/3,CpG_4,CpG_5,CpG_6),CpG_1,CpG_4,CpG_5,CpG_6在三組樣本中的甲基化率分布差異無統(tǒng)計學(xué)意義(p0.05)。CpG_2/3在三組樣本甲基化率分布差異具有統(tǒng)計學(xué)意義(p0.05)。病例組CpG_2/3甲基化率高于陰性對照組,差異有統(tǒng)計學(xué)意義(P0.05),病例組CpG_2/3甲基化率高于正常對照組,差異有統(tǒng)計學(xué)意義(P0.05),但陰性對照組與正常對照組CpG-2/3甲基化率差異無統(tǒng)計學(xué)意義(P0.05)。結(jié)論:Notch4基因甲基化通過炎癥反應(yīng)及信號通路調(diào)控肺纖維的發(fā)生發(fā)展。
[Abstract]:Objective: to investigate the clinical significance of Notch4 gene methylation in peripheral blood of pigeon feeding pigeons in Uygur nationality. Methods: according to the criteria of inclusion and exclusion, the data of Kashi cases and blood samples from 2015 to 2016 were collected. Among them, 20 cases were diagnosed as case group and 20 cases as negative control group. In the same period, 20 Uygur people without pigeon health examination were used as the normal control group. The blood samples were routinely extracted and amplified by hydrogen sulfite transformation PCR, transcribed in vitro and digested with RNase A specific enzyme. Simultaneous matrix assisted laser desorption ionization time of flight mass spectrometry was used to detect Notch4 gene methylation. Results: there was no significant difference in the distribution of methylation rate of Notch4 gene in the three groups by detecting the total number of CpG sites in Notch4 fragments, six CpG1 / CpG1 / CpG1 / CpG1 / CpG4 / CpG4 / CpG5 / CpG5 / CpG5 / CpG5CpG6 in all three groups of samples, and the number of CpG1CpG5CpG6 in the three groups was not significantly different from that of CpG1CpG5CpG6. There was significant difference in the distribution of methylation rate among the three groups. The CpG_2/3 methylation rate in the case group was higher than that in the negative control group. The difference was statistically significant (P 0.05). The rate of CpG_2/3 methylation in the case group was higher than that in the normal control group. The difference was statistically significant (P 0.05), but there was no significant difference in CpG-2/3 methylation rate between the negative control group and the normal control group. Conclusion the methylation of the 1: Notch4 gene regulates the development of lung fiber through inflammatory reaction and signal pathway.
【學(xué)位授予單位】：石河子大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：R563

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