本文選題：鴨　切入點：肌細胞生成素　出處：《湖南農(nóng)業(yè)大學(xué)學(xué)報(自然科學(xué)版)》2017年02期 　論文類型：期刊論文

【摘要】：采用RT PCR技術(shù)擴增和克隆鴨Myo G基因啟動子,并對其啟動子序列進行生物信息學(xué)分析,采用Sequenom Mass Array技術(shù)檢測Cp G島在鴨肌肉組織中的甲基化水平,用q RT PCR檢測Myo G基因的表達量。結(jié)果表明,擴增得到鴨Myo G基因啟動子序列2 730 bp,對啟動子序列預(yù)測后,發(fā)現(xiàn)存在2個Cp G島,其中Cp G島( 2 536~ 1 997 bp)存在5個轉(zhuǎn)錄因子結(jié)合位點和多個真核生物結(jié)構(gòu)元件。甲基化檢測結(jié)果表明:在鴨的個體和組織水平上,啟動子甲基化率均未聚類在一起;Cp G位點甲基化頻率存在個體差異,22%Cp G位點的甲基化頻率與Myo G的m RNA表達量呈負相關(guān)(P0.05),78%Cp G位點的甲基化頻率呈正相關(guān)(P0.05),其中,腿肌甲基化位點Cp G_1、Cp G_26.27.28.29的甲基化頻率與Myo G基因表達水平均呈顯著相關(guān)(P0.05)。Myo G基因在鴨與在哺乳動物中的轉(zhuǎn)錄調(diào)控機制存在差異。試驗中發(fā)現(xiàn)多個影響鴨Myo G基因轉(zhuǎn)錄的潛在甲基化位點,其中Cp G_1與Cp G_26.27.28.29能通過DNA甲基化修飾影響Myo G基因在鴨腿肌中的轉(zhuǎn)錄。本研究結(jié)果可為鴨Myo G基因轉(zhuǎn)錄調(diào)控提供參考依據(jù)。
[Abstract]:The promoter of duck Myo G gene was amplified and cloned by RT PCR, and its promoter sequence was analyzed by bioinformatics. The methylation level of CP G island in duck muscle tissue was detected by Sequenom Mass Array technique. Q RT PCR was used to detect the expression of Myo G gene. The results showed that the promoter sequence of duck Myo G gene was 2 730 bp. after predicting the promoter sequence, two CpG islands were found. There are 5 transcription factor binding sites and several eukaryote structural elements in CpG island (Cp2 536 ~ 1 997 BP). Promoter methylation rate was not clustered together. There were individual differences in methylation frequency of CP G locus. There was a negative correlation between methylation frequency at CP G site and m RNA expression of Myo G. There was a positive correlation between methylation frequency at CpG site and m RNA expression level in Myo G locus, and there was a positive correlation between methylation frequency at CpG locus and m RNA expression level in Myo G locus. The methylation frequency of CP G 26.27.28.29 in leg muscle methylation site was significantly correlated with the expression level of Myo G gene. There were significant differences in the transcriptional regulation mechanism between duck and mammal. Potential methylation sites for transcription, CpG1 and CP G26.27.28.29 can affect the transcription of Myo G gene in duck leg muscle by DNA methylation. The results can provide a reference for the regulation of Myo G gene transcription in duck leg muscle.
【作者單位】： 四川農(nóng)業(yè)大學(xué)動物遺傳育種研究所;
【基金】：國家自然科學(xué)基金項目(31301964) 四川省科學(xué)技術(shù)廳基金項目(2015JY0110)
【分類號】：Q78;S834

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本文編號：1616906