本文選題：桃　切入點：果膠甲酯酶　出處：《江蘇農(nóng)業(yè)科學(xué)》2016年05期 　論文類型：期刊論文

【摘要】：果膠甲酯酶屬于碳水化合物酯酶家族CE-8,是調(diào)節(jié)果膠甲酯化程度的一種普遍存在的酶,是植物細胞壁的主要成分。為深入研究桃PME基因家族的功能,利用生物信息學(xué)方法對桃基因組中PME基因家族成員進行鑒定,并對其基因組信息、蛋白生理生化特征、基因結(jié)構(gòu)、保守結(jié)構(gòu)域和系統(tǒng)進化樹等方面進行了研究。結(jié)果表明:在桃基因組中共鑒定出69個PME基因,可以分為GroupⅠ、GroupⅡ兩大類,GroupⅡ又可以進一步分成3個分支;PME基因在桃的8條染色體上呈現(xiàn)不均勻分布,并存在明顯的串聯(lián)重復(fù)現(xiàn)象,共發(fā)現(xiàn)18個串聯(lián)重復(fù)基因簇,包含47個基因,串聯(lián)重復(fù)是桃PME基因擴增的主要方式。對桃PME基因結(jié)構(gòu)的分析表明,PME基因在進化上存在著一定的保守性;對桃PME蛋白氨基酸序列的多重比對發(fā)現(xiàn)了PME蛋白5個典型的保守結(jié)構(gòu)區(qū)域;通過MEME軟件對桃PME蛋白保守基序的分析發(fā)現(xiàn)了12個比較保守的基序。
[Abstract]:Pectin methylesterase belongs to the carbohydrate esterase family CE-8, which is a ubiquitous enzyme that regulates the degree of pectin methyl ester and is the main component of plant cell wall. In order to study the function of peach PME gene family, pectin methylesterase belongs to the carbohydrate esterase family. The PME gene family members in peach genome were identified by bioinformatics, and the genome information, protein physiological and biochemical characteristics and gene structure were analyzed. The conserved domain and phylogenetic tree were studied. The results showed that 69 PME genes were identified in peach genome. It can be divided into two groups, Group 鈪，

本文編號：1614653